摘要
为探究高海拔生境下怀玉山高山马铃薯的适应机制,本研究以怀玉山高山马铃薯(麻籽洋芋)和怀玉山本土农家薯的采后块茎为试验材料进行转录组分析。结果表明,怀玉山高山马铃薯上调的差异基因956个,下调的差异基因2 262个,总数为3 218个;怀玉山高山马铃薯和怀玉山本土农家薯与叶绿素结合、光系统Ⅰ、光合作用和光捕获、光系统Ⅱ、血红素结合、氧化还原酶活性等光合系统相关的GO term富集显著;怀玉山高山马铃薯和怀玉山本土农家薯与光合作用-天线蛋白、丙烷合成、光合作用、苯丙氨酸代谢等Pathway也富集显著,说明怀玉山高山马铃薯和怀玉山本土农家薯在怀玉山高海拔生境下主要是涉及到光合作用基因的差异表达。本研究结果为怀玉山高山马铃薯适应高海拔生境相关基因的挖掘与应用提供了一定的理论参考。
To explore the adaptation mechanism of alpine potato in Huaiyushan under high altitude habitats,transcriptional analysis was carried out with the tubers of alpine potato and local farm potato in Huaiyushan in this study.The results showed that there were 3 218 differentially expressed genes,among which 956 genes were up-regulated,2 262 genes were down-regulated genes.GO terms associated with chlorophyll binding,photosystemⅠ,photosynthesis and light harvesting,photosystemⅡ,heme binding and oxidoreductase activity in alpine potato and local farm potato in Huaiyushan were significantly enriched.Pathways associated with photosynthesis-antenna protein,propane synthesis,photosynthesis,phenylalanine metabolism alpine potato and local farm potato in Huaiyushan were also significantly enriched,which indicated that in high altitude habitats,the differential expressed genes between alpine potato and local farm potato in Huaiyushan are mainly those related to photosynthesis genes.The results can provide reference for mining and application of the genes related to adaption to high altitude habitats in alpine potato in Huaiyushan.
作者
尹明华
谭鑫
郑亚娇
徐迎昕
邱梦琴
曾清华
蔡红
陈荣华
YIN Minghua;TAN Xin;ZHENG Yajiao;XU Yingxin;QIU Mengqin;ZENG Qinghua;CAI Hong;CHEN Ronghua(College of Life Sciences,Shangrao Normal University,Shangrao,Jiangxi 334001;Red sun Agricultural Development Co.,Ltd.,Shangrao,Jiangxi 334700)
出处
《核农学报》
CAS
CSCD
北大核心
2019年第7期1330-1339,共10页
Journal of Nuclear Agricultural Sciences
基金
国家自然科学基金项目(31860084
31360072)
2014年度江西省高等学校科技落地计划项目(KJLD14086)
关键词
高海拔生境
怀玉山高山马铃薯
怀玉山本土农家薯
块茎
转录组分析
high altitude habitats
alpine potato in Huaiyushan
local farm potato in Huaiyushan
tuber
transcriptome analysis
