摘要
目的 :研究缺氧诱导因子-1α在胆囊癌侵袭转移中的作用,并探讨HIF-1α在判断胆囊癌治疗效果及预测胆囊癌复发转移方面的价值。方法 :采用慢病毒对胆囊癌细胞系(GBC-SD)进行转染,干扰该细胞系中HIF-1α的表达,得到HIF-1α表达不受干扰的胆囊癌细胞系(GBC-SD-NC)和HIF-1α表达受干扰的胆囊癌细胞系GBC-SD-HIF。通过将两组细胞系裸鼠鼠尾静脉注射建立胆囊癌血行转移模型,检测两组细胞在小鼠肺部形成转移瘤的能力。结果 :GBC-SD-NC组裸鼠肺部转移瘤数量、大小都比GBC-SD-HIF组高,GBC-SD-NC组转移瘤的平均数量显著多于GBC-SD-HIF组。结论 :干扰胆囊癌细胞中HIF-1α的表达,可以显著减低胆囊癌细胞系GBC-SD的血行肺转移能力。说明缺氧诱导因子-1α在胆囊癌侵袭转移中存在独立影响。
Objective To study the effects of HIF-1α on the metastasis of gallbladder neoplasm, and discusses the value of HIF-1α on gallbladder neoplasm treatment. Methods Lentivirus infections were carried on to obtain the GBC-SD-NC whose expression of HIF-1α was normal,and GBC-SD-HIF that expression of HIF-1α was interfered. HIF-1α expression was interfered through vena caudalis in nude mice exenografts. HIF-1α expression effect was demonstrated on the metastasis ability of gallbladder neoplasm cells. Results GBC-SD-NC group whose number of metastasis and volume of metastasis were remarkably superior to the GBC-SD-HIF group. In In GBC-SD-NC group, the average number of metastasis foci permouse was marked more than GBC-SD-HIF group. Conclusion The interference of HIF-1α expression could lead to the decline of the metastasis ability of gallbladder neoplasm cells. The Results showed that HIF-1α could produce effects in on the metastasis of gallbladder neoplasm.
出处
《湖南师范大学学报（医学版）》
2015年第2期123-125,共3页
Journal of Hunan Normal University(Medical Sciences)
基金
陕西省科学技术研究发展项目(2014K11-03-03-1)
