摘要
目的:探讨血小板凝血酶蛋白1(THBS1)表达上调对肺腺癌细胞增殖、凋亡和细胞周期的影响。方法:采用实时荧光定量PCR检测肺腺癌细胞株A549、H1299、H358、H1975、PC9、PG49和Calu3中THBS1 mRNA的表达量;构建真核表达载体pc DNA3. 1-THBS1,以脂质体转染至THBS1 mRNA表达量最低的H1975细胞中,实时荧光定量PCR检测THBS1 mRNA表达水平,CCK-8法检测细胞增殖能力,流式细胞技术分析细胞凋亡及细胞周期分布情况。以未转染和转染空质粒pc DNA3. 1的H1975细胞作对照。结果:不同肺腺癌细胞株中THBS1 mRNA表达量不同,H1975细胞中表达最低。与未转染和转染空质粒的H1975细胞比较,转染pc DNA3. 1-THBS1的H1975细胞THBS1 mRNA表达上调(P <0. 05),细胞增殖能力增强(P <0. 05),细胞凋亡率降低(P <0. 05),细胞周期进程被促进(P <0. 05)。结论:THBS1与肺腺癌细胞的生物学行为关系密切,有可能成为肺腺癌的治疗靶点。
Aim: To investigate the effects of thrombospondin 1( THBS1) expression up-regulation on proliferation,apoptosis and cell cycle of lung adenocarcinoma cells. Methods: The expression levels of THBS1 mRNA in lung adenocarcinoma cell lines A549,H1299,H358,H1975,PC9,PG49 and Calu3 were detected by real-time quantitative PCR( qRTPCR) . Eukaryotic expression vector pcDNA3. 1-THBS1 was constructed and transfected into H1975 cells by liposomes. qRT-PCR was used to detect the expression of THBS1 mRNA,CCK-8 kit was used to detect the proliferation, and flow cytometry was used to investigate the apoptosis and cell cycle distribution. The H1975 cells without transfection or transfected with pcDNA3. 1 were the controls. Results: The expression levels of THBS1 mRNA in different lung adenocarcinoma cell lines were different,and the lowest was in H1975 cell line. Compared with the controls, the expression of THBS1 mRNA in H1975 cells transfected with pcDNA3. 1-THBS1 was higher,proliferation ability was increased, the apoptosis rate decreased, and the cell cycle progression was promoted significantly( P < 0. 05) . Conclusion: THBS1 protein is related with the biological behavior of lung adenocarcinoma cells,and may be a treatment target.
作者
王晓慧
刁长英
谢艺林
刘亚清
高献争
韩静
李晟磊
刘红涛
WANG Xiaohui;DIAO Changying;XIE Yilin;LIU Yaqing;GAO Xianzheng;HAN Jing;LI Shenglei;LIU Hongtao(Department of Pathology, the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052;Laboratory for Cell Biology,College of Life Sciences,Zhengzhou University,Zhengzhou 450001)
出处
《郑州大学学报（医学版）》
CAS
北大核心
2018年第6期730-733,共4页
Journal of Zhengzhou University(Medical Sciences)
基金
国家自然科学基金资助项目(81372677)
河南省基础与前沿项目(132300410073)
河南省自然科学基金资助项目(182300410377)
河南省高等学校重点科研项目(17A180016)
关键词
血小板凝血酶1
肺腺癌
细胞增殖
细胞凋亡
细胞周期
thrombospondin 1
lung adenocarcinoma
cell proliferation
cell apoptosis
cell cycle
