摘要
采用Agilent 110 0液相色谱仪测定斑节对虾血淋巴中诺氟沙星含量 ,并初步研究了斑节对虾一次性肌肉注射诺氟沙星的药代动力学 ,斑节对虾血淋巴药 时曲线可以用开放性二室模型来描述。由此推算诺氟沙星的药动学参数 ,分布和消除半衰期分别为 0 .0 6 35和 0 .6 12h ;曲线下面积 (AUC)、总体消除率 (CLs)和表观分布容积 (Vd)分别为 12 .42 5ug·h/mL、80 4.83mL/kg·h和 710 .35mL/kg。
A RP-HPLC method was developed to determine the norfloxacin hemolymph concentration of the giant tiger shrimp, Penaeus monodon. HPLC analysis was performed by Agilent 1100. The column was Agilent ZORBAX SB-C18. The mobile phase consisted of acetonitrile:phosphate buffer(10 mmol/L sodium phosphate monobasic and 5 mmol/L tetrabutylammonium bromide)(14.5:85.5,V/V). The flow rate 0.5 mL min -1, the UV detector wasset at 280 nm, and the column temperature was 25℃. The linear range of norfloxacin was 0.051-20.4 ug/mL, the recovery of norfloxacin from hemolymph was 96.49±1.60%, and theintra-day and inter-day precision were under 3%. The RP-HPLC method could be adapted to determine the norfloxacin concentration in hemolymph and was used to briefly study the pharmaokinetics of norfloxacin in the shrimp. A two-compartment open pharmacokinetic model was used to describe the norfloxacin hemolymph concentration-time profile and the pharmacokinetic model was C 0=11.73×e -1.133t+22.62×e -10.918t.The model-derived pharmacokinetic parameters were gained. The distribution half-life (t α 1/2) and elimination half-life(t β 1/2) were 0.0635 and 0.612 h, respectively ; the area under the concentration versus time curve(AUC), total body clearance (CLs) and apparent volume of distribution (Vd) were 12.425 ug·h/mL?804.83 mL/kg·h and 710.35 mL/kg.
出处
《水生生物学报》
CAS
CSCD
北大核心
2003年第1期13-17,共5页
Acta Hydrobiologica Sinica
基金
农业部重点科研项目 (渔 95-B- 0 0- 0 1- 0 5)
