摘要
OBJECTIVE: To demonstrate the changes in gap junctional intercellular communication (GJIC) mediated by low power density microwave radiation in rabbits lens epithelial cells (LECs) and its mechanisms. METHODS: Rabbits' eyes were exposed to 5 mW/cm(2) and 10 mW/cm(2) power densities of microwave radiation for 3 hours. The fluorescence-recovery-after-photobleaching (FRAP) method was used to determine the GJIC. The localization and function of connexin 43 in LECs was detected by laser scanning confocal microscopy. RESULTS: The GJIC of rabbits LECs was inhibited by microwave radiation especially in the 10 mW/cm(2) irradiated samples. A decrease in connexin 43-positive staining was seen in 5 mW/cm(2) x 3 h treated LECs. Intracellular space accumulation and cytoplasmic internalization were clearly demonstrated in 10 mW/cm(2) group. CONCLUSIONS: Low power densities microwave radiation (5 mW/cm(2) and 10 mW/cm(2)) induces damage to connexin 43 and inhibits the GJIC of rabbits LECs. These changes result in an osmotic imbalance within the lens and induce early cataract. 5 mW/cm(2) or 10 mW/cm(2) microwave radiation is cataractogenic.
目的 研究低强度微波辐射对兔眼晶状体上皮细胞细胞间隙连接通讯功能的影响及其机理。方法 健康家兔接受 3h 5mW/cm2 或 10mW/cm2 微波辐射。取兔双眼晶体前囊膜 ,用 5 ,6 羟基荧光素乙酸乙酰盐染色作激光共聚焦显微镜晶体上皮细胞荧光淬灭及恢复情况测定。用免疫荧光法标记Cx4 3作定位检测。结果 接受 5mW /cm2 或 10mW /cm2 微波辐射后兔眼晶体上皮细胞荧光恢复速度明显减慢 ,GJIC功能破坏。Cx4 3荧光标记定位结果显示 ,5mW/cm2 实验组Cx4 3阳性膜表达减少 ;10mW/cm2 实验组出现Cx4 3的细胞外聚集 ,胞浆和细胞核内位移。结论 5mW/cm2 和 10mW/cm2 低强度微波通过破坏Cx4 3的正常功能定位而抑制晶体上皮细胞间GJIC功能 ,破坏晶体内稳态 ,促使晶体上皮细胞发生凋亡 ,诱发白内障。
