摘要
本实验研究了吉富罗非鱼(Oreochromis niloticus)大豆粕日粮中添加植酸酶替代磷酸二氢钙的养殖效果。实验设计了共7组实验组日粮,其中实验组1日粮主要蛋白原料为鱼粉;其它6个实验组日粮以大豆粕为主要蛋白原料,植酸酶添加量为日粮的0.1%(酶活性为2000U/g),日粮中分别添加2.5%、2.0%、1.5%、1.0%、0.5%和0%的磷酸二氢钙。每组日粮设计3个平行,每个平行20尾鱼[(1.9±0.1)g],随机饲养于室内水泥池网箱中,实验期为60d。实验结果表明,7个实验组鱼存活率之间没有显著性差异(P>0.05);鱼粉日粮组鱼的生长效果与饲料效益、全鱼、鳞片、骨钙、磷含量和粪便磷含量均显著高于大豆粕组鱼(P<0.05);实验组2、3、4鱼的特定生长率、脏体指数、肠脂指数、肥满度、鱼体近似值成分无显著性差异(P>0.05);实验组2、3、4鱼的全鱼、鳞片和鱼骨钙、磷含量无显著性差异(P>0.05),但均显著高于实验组5、6、7鱼(P<0.05)。实验结果表明,在以大豆粕为主植物蛋白原料的吉富罗非鱼日粮中添加植酸酶能够增加日粮中的有效磷,减少日粮中40%左右的磷酸二氢钙添加。
The experiment was performed to test the effect of using phytase to replace monocalcium phosphate in the soybean meal diets of GIFT Nile tilapia(Oreochromis niloticus). Seven formulae of diets were designed. Formula 1 was made with fish meal as the main protein supplement, and the other 6 groups were made with soybean meal as the main protein source with adding of 0.1% phytase(enzyme activity was 2000U/g), and 2.5%, 2.0%, 1.5%, 1.0%, 0.5%, and 0 of monocalcium phosphatein diets, respectively. Each diet was triplicated, each with 20 fish [(1.9±0.1)g] reared in indoor cement pool cage. The experiment lasted for 60 days. Results showed no significant difference in survival rate(P>0.05) among 7 experimental groups. The effects of growth, feed efficiency, calcium content and phosphorus content of whole fish, fish scales and vertebral bone, and the fecal phosphorus content of fish meal group were significantly higher than those of soybean meal groups(P<0.05). In addition, no significant difference was shown in specific growth rate, viscerasomatic indices, intestinalsomatic indices, condition factor and body approximation components among experimental Formulae 2—4(P>0.05), and nor did in calcium, phosphorus content of whole fish, fish scales and vertebral bone(P>0.05), but significantly higher than those of Formulae 5—7(P<0.05). Therefore, adding phytase in tilapia diets of soybean meal can increase effective phosphorus, and reduce for about 40% monocalcium phosphate adding into diet.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2014年第5期1051-1057,共7页
Oceanologia Et Limnologia Sinica
基金
广西科技厅攻关项目资助
122201-3号
关键词
吉富罗非鱼
植酸酶
磷酸二氢钙
植物蛋白
钙磷沉积
Oreochromis niloticus
phytase
monocalciumphosphate
plant protein
calcium and phosphorus deposition
