摘要
OBJECTIVE: To study the effect and mechanism of the peripheral blood mononuclear cell (PBMC) invasion by HBV on artificial immunization in newborns. METHODS: Fifty-two newborns of HBsAg positive mothers were immunized with HBIG (hepatitis B immunoglobulin) and HBVac (hepatitis B vaccine) and were followed up for 7 months. The newborns' HBV-DNA in serum and in the PBMCs was detected with nested-PCR; anti-HBs was tested with solid phase radioimmunoassay (SP-RIA). PBMCs isolated from newborn peripheral blood were incubated in the presence of PHA or purified HBsAg. Interleukin-2 (IL-2) level in culture supernatants of activated cells was detected by ELISA. RESULTS: The failure rate of immunization was higher in infants with positive HBV-DNA in PBMCs than those with negative HBV-DNA (P
目的 探讨HBV侵犯外周血单个核细胞 (PBMC)对新生儿免疫接种的影响 ,并从IL 2角度分析其可能机制。方法 5 2例HBsAg阳性母亲新生儿分娩后用HBIG和HBVac联合免疫接种 ,7月龄时进行随访。Nested PCR技术检测新生儿血清和PBMC中HBVDNA ,固相放免法检测血清抗 HBs。采用体外细胞培养和双抗体夹心ELISA法检测新生儿PBMC在PHA和HBsAg刺激下培养上清液中IL 2的含量。结果 HBV宫内侵犯PBMC后 ,新生儿免疫接种失败率显著高于PBMC未受侵犯组 (P <0 0 5 ) ;新生儿PBMC培养上清液中IL 2含量显著低于未受侵犯组和正常对照 (P <0 0 5 )。免疫接种失败组新生儿PBMC培养上清液中IL 2含量明显低于免疫接种成功组和正常对照 (P <0 0 5 )。结论 HBV宫内侵犯PBMC是新生儿免疫接种失败的重要原因。HBV侵犯PBMC可使其IL 2分泌能力下降 ,这可能是导致新生儿免疫接种失败的机制之一。
