摘要
OBJECTIVE: To evaluate the role of thrombopoietin (TPO) in the pathology of chronic thrombocytopenic disease. METHODS: We measured the endogenous plasma concentration of TPO in 40 patients with acquired aplastic anaemia (AA) and in 32 patients with idiopathic thrombocytopenic purpura (ITP) by a sensitive Sandwich enzyme-linked immunosorbent assay (ELISA) and compared the results. RESULTS: Plasma TPO concentrations were significantly higher in AA patients (774 +/- 393 pg/ ml) in comparison with healthy control subjects (55 +/- 34 pg/ml, P 0.05). There was also no relationship between their plasma TPO levels and platelet counts. CONCLUSIONS: TPO levels may be regulated not only by platelets but also by megakaryocytes in AA and ITP, and measurement of TPO levels is useful for diagnosing thrombocytopenia and understanding the pathophysiology of thrombocytopenia.
目的 为了研究血小板生成素 (TPO)在慢性血小板减少性疾病中的病理变化。方法 我们用敏感的双抗体夹心法酶联免疫吸附试验 (ELISA)检测了 4 0例再生障碍性贫血 (AA)和 32例特发性血小板减少性紫癜 (ITP)病人的血浆TPO的浓度。结果 AA患者血浆TPO浓度 (774± 393pg/ml)明显高于正常人 (5 5± 34pg/ml,P <0 0 0 1) ,并且与血小板计数呈负相关 ;ITP患者血浆TPO水平正常或仅轻度增高 (73± 36pg/ml) ,与正常人相比无显著性差异 (P >0 0 5 ) ,其血浆TPO浓度与血小板计数间也无相关性。结论 AA和ITP患者血浆TPO含量不仅受到外周血血小板同时也受到骨髓巨核细胞的调节。血浆TPO含量的检测有助于血小板减少性疾病的诊断及病理研究。
