摘要
[目的]研究神经元轴突损伤后再生过程中对神经元胞体内脂褐素生成和累积的变化,了解神经再生对衰老进程的影响。[方法]钳夹法损伤Wistar大鼠右侧C_5～T_1神经根,按钳夹次数和存活时间分为6组,观察损伤侧脊髓前角神经元内脂褐素变化,用图像分析系统进行定量分析。[结果]12月龄大鼠脊髓前角神经元胞浆内已有脂褐素分布,自发出黄或黄绿色荧光,呈散在细小颗粒,含量呈随增龄而增加,且呈团块状堆积在核周围。钳夹侧的脂褐素含量较正常侧的脂褐素含量均有明显减少(P<0.05);二次钳夹后脂褐素含量较一次钳夹更为减少,但二者之间无明显差异(P>0.05)。直线相关回归分析显示正常侧、一次钳夹侧和二次钳夹侧脂褐素累积均随存活时间而增加,呈正相关关系(γ分别为0.967、0.994和0.995,P< 0.05),回归系数分别为0.0783、0.0548和0.0377。钳夹侧小于正常侧,表明损伤后再生使胞体内脂褐素累积速度减慢。[结论]轴突损伤后再生可以使神经元内脂褐素的累积较正常情况下减少,提示轴突再生可减缓神经元衰老进程。
[Objective] To observe the changes in the formation and deposit of lipofuscin during axonal regeneration after axonal lesion and study the effect of regeneration on aging. [Methods] C5-T1 nerve roots of right brachial plexus of Wistar rats were crushed, and all animal models were divided into 6 groups by crush times and surviveal time. The amount changes of lipofuscin in the injuryed anterior horn neurons were observed by image analysis system. [Results] Lipofuscin existed in the plasm of the spinal cord anterior horn neurons of rats aged 12 months, which appeared as micro-granules. The autofluorescence was yellow and chryso-green. The amount of lipofuscin increased with aging, accumulating as a mass in the perinuclear region, but decreased remarkably after the crushes ( P < 0.05 ) , However the difference was no significant between the first and the second crush. The relation and regression analysis showed that the deposit of lipofuscin increased gradually as a function of survival time on either the normal side, one crush side or two crushes side, which showed a positive correlation (The values of r were 0.967, 0.994 and 0.995,respctively P <0.05). The regression coefficients were 0.0783, 0.0548 and 0.0377, respectively. [Conclusion] The axonal regeneration can decrease the deposit of lipofuscin and make it less than that of normal condition, which suggests the axonal regeneration can delay the process of neuron aging.
出处
《中山医科大学学报》
CSCD
北大核心
2003年第1期16-18,34,I002,共5页
Academic Journal of Sun Yat-sen University of Medical Sciences
基金
"211工程"重点学科建设基金(98123)
