摘要
[目的]为建立一种简便、实用的 DYF155S1位点多态性分析技术提供依据。[方法]采用扩增片段长度多态性(Amp-FLP)方法,调查中国汉族人群155个无关男性个体DYF155S1位点长度的变异情况。PCR扩增产物用非变性聚丙烯酰胺凝胶电泳,银染显示条带。用循环测序法对该位点的10个等位基因进行正向和反向序列分析。[结果]以基因组 DNA为模板,用一对引物可同时扩增出 DYF155S1和DYF155S2 2个位点的等位基因。DYF155S1位点表现出有长度多态性,这些等位基因约 1500～2 500 bp。DYF155S2位点表现出有或缺失的二态现象,缺失率约为7.1％。发现有4种变异的核心序列,其中3种与文献报道一致,被命名为1型、3型和4型,另一种为新发现的变异核心序列,暂命名为7型。10个等位基因具有相同的模块结构,在5’端表现为3型-1型-3型排列,在3’端则表现为 4型-3型排列。[结论]中国汉族人群DYF155S1位点 5’端序列较3’端的多态性高。
[Objective] To establish a simple and useful method for polymorphism analysis of DYF155S1 locus(NSYl locus). [Methods] The allele length variation of DYF155S1 locus from 155 unrelated males in Chinese Han population was studied by using amplified fragment length polymorphism (Amp-FLP) method. PCR products were detected by using 6% polyacrylmide gel electrophore-sis followed by silver staining. Ten alleles at DYF155S1 locus were sequenced from 5' and 3'ends by Cycle Sequencing. [Results] DYF155S1 and DYF155S2 loci could be amplified simultaneously from genomic DNA by using a couple of primers. The alleles at DYF155S1 locus showed length polymorphism and their sizes ranged from 1500 to 2 500 bp. While the alleles at DYF155S2 locus showed yes/ no deletion polymorphism and the rate of deletion was 7.1%. Four types of variant repeats were identified, including three types, designated Type 1, 3 and 4 that reported before whereas one type, named Type 7, was a new found. Ten alleles sequenced had the same modular structure, starting with a repeat block of Type 3 followed by a block of Type 1 before the central block of Type 3 at 5'end, and starting with a repeat block of Type 4 followed by the central repeat block of Type 3 at 3'end. [Conclusion] The polymorphism of DYF155S1 locus at 5'end in Chinese Han population is higher than at 3' end. Our results provide the basis for analyzing the polymorphism of DYF155S1 locus.
出处
《中山医科大学学报》
CSCD
北大核心
2003年第1期42-45,共4页
Academic Journal of Sun Yat-sen University of Medical Sciences
基金
中山医科大学"211"工程科研基金(98022)
