摘要
选用分布于水稻 1 2条染色体上的 2 6对 SSR引物对我国 9个主要的杂交水稻组合及其亲本进行了 SSR标记分析 ,2 1对多态性引物共扩增出 62条条带 ,平均 2 .95条 ,能够有效地区分所有恢复系和大部分不育系。杂交种条带均为父母本的互补型 ,很适合做杂交种纯度鉴定。用引物 RM1 7对杂交稻组合汕优 63和两优培九进行了 1 0 0粒单种子 SSR鉴定 ,所测纯度分别为 96.0 %和 98.0 % ,与田间纯度 96.2 %和 97.7%非常接近 ,显示出 SSR技术在品种认证和纯度鉴定中有广阔的前景。
Twenty six SSR primers distributing in 12 rice chromosomes were used to detect nine major hybrid rice combinations and their parents in China. Out of the primers used, 21 were polymorphic and amplified 62 bands totally with a mean of 2.95 bands per primer. It could distinguish all restorer lines and most sterile lines effectively. The bands of hybrid rice were complement types of their parents, which were adapted to examine purity of hybrid rice seed. Primer RM17 was selected for SSR identification with 100 single seeds of Shanyou 63 and Liangyoupeijiu. The purity was 96.0% and 98.0%, respectively, which were approximate to their field purity (96.2% and 97.7%, respectively). It suggested that SSR technique has wide prospective in variety authentication and purity identification.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2003年第1期1-5,共5页
Chinese Journal of Rice Science
基金
国家农业科技跨越计划 (1 999- 0 1 - 1 5)
农业部水稻学重点实验室开放项目(0 0 0 2 0 3)
