摘要
目的 探索成年哺乳动物心肌细胞分离、培养技术。方法 依次用低钙、蛋白酶及胶原酶灌流兔心脏 ,分离心肌细胞 ,并用悬浮法和贴壁法进行无血清原代培养。结果 新分离心肌细胞的产率为 5× 10 6 个 ,其中长杆状细胞占 85 %。用悬浮法培养 4～ 5天 ,细胞保持完整的形态结构 ,用贴壁法细胞可培养数周。结论 此法是比较理想的心肌细胞分离。
Objective To investigate an effective method to isolate and cultivate adult mammalian ventricular cardiac myocytes. Methods Rabbit cardiac myocytes were isolated with low calcium, protease and collagenase successively and the myocytes were cultured with suspending and adherent method. Results The total amount of the cardiac myocytes obtained was 5×10 6, and over 85% elongated cross-striated myocytes was obtained. The cells can keep its structure and morphology in 4-5 days using suspending method and can be kept in long time culture using adherent method. Conclusion The results suggest that the methods we developed for isolation and culture of adult rabbit cardiac myocytes are ideal for the isolation and culture of cardiac myocytes.
出处
《徐州医学院学报》
CAS
2003年第1期20-22,共3页
Acta Academiae Medicinae Xuzhou
基金
江苏省教委自然科学基金资助课题 (0 2KJB0 0 0 8)
关键词
心肌细胞
细胞培养
细胞分离
cardiac myocytes
cell culture
cell isolation
