摘要
以低等真核生物的5.8S rRNA为材料进行研究。发现在用今祖法消除了进化速度之差的影响所得到的差异矩阵d'中,各组理应彼此相等的数值会因核苷酸偶合的影响而成为不等值的。取各组中各自的的最大值作为受偶合的影响最小者,以之对今祖法所得到的分枝型式进行校正,即可得到更为正确的结果。在此基础上提出了对差异矩阵d'进行最大值成聚以代替原法中的平均值成聚的削偶合今祖法。新法在结果的正确性、稳定性和一致性上都优于原法。
The sequence data used were the conservative part of 5.8S rRNAs of seven species of protists (yeast, ciliate, trypanosomatid, microspora, diplomonad and two species of dinoflagellates) and of one species of higher plant (rice). The corresponding part in 23S rRNAs of seven species of prokaryotes (eubact-erium, halophilic archaebacteria, methane-producing archaebacteria and sulfur-dependent thermophilic archaebacteria) were separately or integrately used as references.
关键词
今祖法
分子进化
分子进化树
Constructing molecular evolutionary tree, Present-day ancestor (PDA) method, Coincidences between homologous macromolecules, Unicellular eukaryotes, 5.8S rRNA