期刊文献+

HPLC法测定复康宝胶囊中补骨脂素与异补骨脂素的含量 被引量:9

Determination of Psoralen and Isops oralen Contents in Fukangbao Capsul e by HPLC
下载PDF
导出
摘要 目的建立反相HPLC法测定康复宝胶囊中补骨脂素、异补骨脂素的含量。方法液相ODS—C18色谱柱,流动相为甲醇-水(40∶60),柱温35℃,流速1mL/min,检测波长247nm。结果补骨脂素和异补骨脂素分别在1.05~10.50μg/mL,1.02~10.20μg/mL范围内线性关系良好,相关系数分别为r补骨脂素=0.9999,和r异补骨脂素=0.9999。两组分的回收率分别是补骨脂素97.5%,异补骨脂素100.8%,RSD均为0.6%。结论本法可同时测定补骨脂素与异补骨脂素,方法简便,精确,为其限量标准提供了科学依据。 Objective To establish a reversed -phase HPLC m ethod for the determination of psora len and isopsoralen in Fukangbao capsule.Methods The contents of Psoralen and Isopsoralen were assayed on a ODS -C 18 column with a mobile phase of methanol -water(40∶60)at a column temperature of 35℃.The fl ow rate was 1.0mL /min.The detection wave-length was at 247nm.Results The linear ranges of Psoralen and Iso psoralen were 1.05~10.52μg /mL(r =0.9999)and1.02~10.20μg /mL(r =0.9999)respectively.Their recoveries were within 97.5%(Psoralen)and 100.8%(Isopsoralen),and both of their RSD were 0.6%.Conclusion This method is simple,rapid and accu rate and suitable for quantity -limiting control of Fukan gbao capsule.
出处 《中药新药与临床药理》 CAS CSCD 2003年第1期39-40,共2页 Traditional Chinese Drug Research and Clinical Pharmacology
关键词 复康宝胶囊 化学 补骨脂素 高压液相色谱法 中药 含量 Fukangbao Capsules /chemictry Psoralen /analysis Chromatography,high pressure liquid
  • 相关文献

参考文献7

二级参考文献23

共引文献149

同被引文献35

引证文献9

二级引证文献48

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部