摘要
含黄嘌呤脱氢酶的细胞用可见光固化树脂包埋 ,经可见光照射交联 ,制备了固定化细胞 .可见光照射 3min对细胞的存活和细胞中黄嘌呤脱氢酶的活性没有影响 .固定化细胞的黄嘌呤脱氢酶降解次黄嘌呤的最适温度为 35℃ ,最适pH为 8 0 .在pH6— 8,温度低于 40℃时稳定 .连续使用 10批 ,平均降解次黄嘌呤 99 19%,酶活力保留 94 99%.
Applying the visible photopolymerisable resin as the carrier, the Arthrobacter ureafaciens cells were entrapped. The visible light irradiation did not affect effect on the survivability of the cells and the activity of xanthine dehydrogenase. For the degradation of hypoxanthine, the optimal temperature and pH were 35℃ and 8.0 respectively. The xanthine dehydrogenase of the immobilized cells was stable when the pH was in the range of 6.0—8.0 and at temperature below 40℃.The remained activity of the enzyme was 94.99% after ten batches of operation for degradation of hypoxanthine,and the average degradation rate of hypoxanthine was 99.19%.
出处
《环境科学学报》
CAS
CSCD
北大核心
2003年第1期74-78,共5页
Acta Scientiae Circumstantiae
