摘要
以转基因Fe-SOD,Mn-SOD高表达、转基因Mn-SOD反义低表达烟草及非转基因品系为供试材料,比较CO2光强对烟草光合作用和蒸腾作用的影响及不同叶位叶片的光合差异.在相同条件下,Mn-SOD高表达烟草CO2补偿点最高(105μmol·mol-1),Mn-SOD低表达烟草最低(78μmol·mol-1),CO2浓度对非转基因烟草蒸腾作用的影响比对其他三个转基因品系的影响要强.Mn-SOD低表达烟草的光补偿点最高(45μmol·m-2·s-1),而Mn-SOD高表达烟草最低(25μmol·m-2·s-1),并且强光对非转基因烟草的光合作用抑制大,转基因品系对强光具有较强的耐受力.同一叶位叶片,转基因烟草的光合速率、蒸腾速率更强.但转基因SOD高表达烟草在整体上并没有表现出明显的光合作用优势.
The effect of CO2,PAR and leaf position on photosynthetic characteristics of transgenic tobacco with Fe-SOD gene or Mn-SOD gene and nontransgenic tobacco are analysised.The results showed:(1)Transgenic tobacco with Mn-SOD gene overexpression in chloroplast had the highest CO2 compensation point(105?μmol·mol-1),transgenic tobacco with Mn-SOD mRNA antisense underexpression had the lowest CO2 compensation point(78?μmo·mol-1). CO2 concentration affected the transpiration rate more in the nontransgenic tobacco than in the transgenic tobacco.(2) Transgenic tobacco with Mn-SOD mRNA antisense underexpression had the highest light compensation point(45?μmol·m-2·s-1),transgenic tobacco with Mn-SOD gene overexpression in chloroplast had the lowest light compensation point(25?μmol·m-2·s-1).The transgenic tobacco had better tolerance to intense light.(3) For the same position leaves,transgenic tobacco had stronger photosynthetic rate and transpiration rate.However,the transgenic tobacco did not show advantages in photosynthesis in general.
出处
《云南大学学报（自然科学版）》
CAS
CSCD
2003年第1期69-72,共4页
Journal of Yunnan University(Natural Sciences Edition)
基金
云南省人才基金资助项目(转基因SOD高表达烟草近等基因系利用价值).
