摘要
采用柱前衍生反相高效液相色谱法 (RP HPLC)测定碱性氨基酸含量 ,衍生化试剂为 2 ,4 二硝基氯苯 (CDNB) ,流动相为 0 .2mol/L的醋酸 醋酸盐缓冲液 ( pH 5 .2 0 )和甲醇的混合溶液 ,其体积配比为 75∶2 5 ,不需梯度洗脱 ;采用C18柱 ,在波长 36 0nm条件下进行紫外检测 ,碱性氨基酸质量浓度与峰面积呈良好的线性关系 ,相关系数均在 0 .98以上 ,分离效果较好 .
A method for determination of basic amino acids by RP HPLC with 2,4 dinitrochlorobenzene precolumn derivation has been developed. Chromatographic conditions were as follows : mobile phase-0.2 mol/L acetate buffer (pH 5.20) and methanol in a ratio of 75∶25; separation column C 18 ; detection wavelength 360 nm. Good linear relation between the concentration of basic amino acids and peak area was obtained, and correlative coefficient was over 0.98. Basic amino acids were well separated by the method.
出处
《无锡轻工大学学报（食品与生物技术）》
CSCD
北大核心
2003年第1期74-76,81,共4页
Journal of Wuxi University of Light Industry
关键词
碱性氨基酸
柱前衍生化
反相高效液相色谱
测定
basic amino acids
2,4 dinitrochlorobenzene
precolumn derivatization
RP HPLC
