摘要
比较了小分子多肽SDS PAGE的各种显色方法 ,结果表明 ,考马斯亮蓝R 2 5 0染色需要 3μg以上的样品 ,相对分子质量 30 0 0左右的肽才能得到清晰条带 ,而对含量低的基因工程产物不能显色 .银染可用于检测微量的分子多肽 ,银染过程中用戊二醛和甲醛进行敏化而减少固定时间 ,可显著提高检测灵敏度 。
This article described several blotting methods of SDS polycrylamide gel electrophoresis to analysis low molecular weight polypeptides. The result showed that when the coomassie brilliant G 250 was used to stain the gel, the band of the peptide with 3 000 molecular weight couldn't be seen clearly unless its mass is above 3 microgramme. During the process of silver blotting the Glutaraldenyde and the Formaldehyde were used to sensitize the stain process, to reduce the time to fix the polypeptides. The sensitivity was significantly improved to detect low molecular peptides by this method. Thus, silver blotting is anexcellent way to detect the tiny amount low molecular peptides produced by recombined microorganism.
出处
《无锡轻工大学学报（食品与生物技术）》
CSCD
北大核心
2003年第1期93-95,共3页
Journal of Wuxi University of Light Industry
基金
国家"十五"科技攻关项目 (2 0 0 1DEA2 0 0 2 2 )
