摘要
目的 :建立准确测定杜仲叶中氯原酸含量的方法 ,为下一步氯原酸提取工艺的研究打下基础 .方法 :采用紫外分光光度法及薄层色谱扫描法分别测定 .结果 :纸层析紫外分光光度法 (剪洗法 )测得杜仲叶中氯原酸含量为 1.94 9% ,变异系数 (CV) 1.34% ,回收率 10 0 .4 4 % .薄层色谱扫描法测得含量为 1.886 % ,变异系数 1.72 % ,回收率 10 1.2 7% .结论 :纸层析紫外分光光度法是测定杜仲叶中氯原酸含量的较优方法 .
AIM: To provide a way for the accurate determination of the chlorogenic acid from the leaf of Eucommia ulmoides Oliv. , and to laya foundation for further studying chlorogenic acid's extraction process. METHODS: UV spectrophotometry and thin layer chromatography (TLC) scanning were used respectively. RESULTS: By paper chromatography UV spectrophotometry the content of chlorogenic acid was 1.949% , CV was 1.34%, and the rate of recovery of purity was 100.44%. By TLC scanning the content was 1.772% , CV was 2.05%, and the rate of recovery of purity was 101.40%. CONCLUSION: Paper chromatography UV spectrophotometry is a better way in the determination of chlorogenic acid content from the leaf of Eucommia ulmoides Oliv. .
出处
《第四军医大学学报》
北大核心
2003年第1期78-80,共3页
Journal of the Fourth Military Medical University
