摘要
目的 测定哮喘患者血浆及豚鼠哮喘模型血浆、肺组织NO及TNF -α的含量 ,观察豚鼠哮喘模型给予皮质激素地塞米松治疗后及L -NAME干预后NO及TNF -α的含量变化 ,了解NO及TNF -α在哮喘发病中的作用 ,探讨其相互关系。方法 采用镉还原法测定血浆、肺组织NO代谢产物 ,应用放免法测定TNF -α含量。结果 哮喘患者血浆及豚鼠哮喘模型血浆、肺组织NO及TNF -α含量均显著高于对照组 (P <0 0 1) ,豚鼠哮喘模型给予皮质激素及L -NAME干预后NO及TNF -α的含量显著低于哮喘模型组 (P <0 0 1)。结论 NO及TNF -α在哮喘发病中起重要作用 ,哮喘患者血浆及豚鼠哮喘模型血浆、肺组织中NO的升高可能与TNF -α产生增多有关。豚鼠哮喘模型应用皮质激素后NO及TNF -α降低表明皮质激素可抑制哮喘时TNF -α生成 ,进而阻止在其刺激下NO的生成与释放。应用NOS抑制剂L -NAME后TNF -α含量亦降低至接近对照组水平 ,提示L -NAME可能抑制过度的TNF -α的生成 ,进而减轻TNF -α诱导的一系列炎症反应。
Objective To investigate the role of nitric oxide (NO) and tumor necrosis factor-α(TNF-α) in the pathogenesis of asthma. Methods We detected the contents of NO and TNF-α in the plasma of asthmatic patients and in the plasma and lung tissue of guinea-pig asthmatic models. As well, we detected the contents of NO and TNF-α in the plasma and lung tissue of guinea-pig asthmatic models after the treatment of the dexamathasone and L-NAME. NO was detected by chemiluminescence and TNF-α was detected by radioimmunity. Results The contents of NO, TNF-α in the plasma of the asthmatic patients and in the plasma and lung tissue of guinea-pig asthmatic models were significantly increased, compared with the control group(P<0.01). These data of guinea-pig asthmatic models in the plasma and lung tissue were significantly decreased after using dexamethasone by inhalation and intraperitoneal injection or L-NAME by intraperitoneal injection(P<0.01). Conclusions NO, TNF-α might play an important role in the pathogenesis of asthma, and the formation of NO might be stimulated by TNF-α, L-NAME and dexamethasone might inhibit the formation of TNF-α, so that it might inhibit and release the formation of the NO in the asthma.
出处
《中国急救医学》
CAS
CSCD
北大核心
2003年第2期76-77,共2页
Chinese Journal of Critical Care Medicine
