摘要
目的 探讨舌鳞癌预后与细胞增殖和凋亡的关系。方法 采用TUNEL法和免疫组织化学法检测 52例舌鳞癌细胞中自发性凋亡水平及Bax、Bcl 2、增殖细胞核抗原 (proliferatingcellnuclearantigen ,PCNA)的表达 ,及其与预后的关系。结果 舌鳞癌中凋亡指数 (apoptosisindex ,AI)、Bcl 2、Bax和PCNA表达的阳性率分别为 55 .8%、65 .4 %、73 .1 %和 1 0 0 .0 %。单因素生存分析显示 :舌癌患者生存率降低与Bax低表达 (P =0 .0 0 2 0 )、PCNA高表达 (P =0 .0 1 79)、Bcl 2 /Bax比值 >1 (P =0 .0 0 72 )和高TNM分期 (P =0 .0 35 1 )有关 ,但与Bcl 2表达和凋亡指数无关 (P =0 .885 7,P =0 .353 6)。结论 Bax、PCNA、Bcl 2 /Bax比值和TNM分期一样具有预后价值 。
Objective To study the correlation between prognosis and cell proliferation, apoptosis in squamous cell carcinoma(SCC)of tongue. Methods Using TdT mediated dUTP biotin nick end labeling (TUNEL) for cell apoptosis and immunohistochemical staining for proliferating cell nuclear antigen (PCNA), Bcl 2 and Bax protein expression. Results In SCC of tongue, the positive rates of apoptosis index(AI)?Bcl 2?Bax and PCNA were 55.8%, 65.4%, 73.1% and 100.0% respectively. In the univariate Kaplan Meier analysis, low expression of Bax, high expression of PCNA,Bcl 2/Bax expression ratio>1 and high staging were all associated with lower survival rate (Log rank test, P <0.05, respectively), AI was only correlated with grading. Bcl 2 did not show a significant association with all features. Conclusions The levels of Bax and PCNA expression and Bcl 2/Bax expression ratio have independent prognosis value in squamors cell carcinoma of the tongue. Comprehensive analysis of these parameters can be helpful to identify biological behavior and predict more accurately the prognosis of patients of tongue squamors cell carcinoma.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2003年第1期31-34,I003,共5页
Chinese Journal of Stomatology
基金
广西壮族自治区自然科学基金资助项目(桂科自9912 0 34 )
关键词
舌鳞癌
预后
细胞增殖
增殖细胞核抗原
细胞凋亡
Tongue
Carcinoma,squamous cell
Prognosis
Proliferating cell nuclear antigen
