摘要
直接从死亡大熊猫肝脏提取细胞总 Ｒ Ｎ Ａ，经反转录后用犬瘟热病毒的 １ 对引物扩增出了约 ３２０ ｂｐ 的片段。此产物经纯化、序列分析表明，其片段 ２ 个引物间长度为 ２８１ ｂｐ，与预计片段大小相同。此毒株在核苷酸和氨基酸水平与北京犬等 ４ 个野毒株、哈尔滨犬野毒株、某疫苗弱毒株、 Ｏｎｄｅｒｓｔｅｐｏｏｒｔ 弱毒株和海豹瘟热病毒 ２ 型毒 株的 同源 性分 别为 ９２２％ 和 ９８９％ 、９２５％ 和 ９８９％ 、９１５％ 和 ９４６％ 、９２９％ 和 ９８９％ 、９８２％ 和 １００％ 。这样就进一步确定了大熊猫的犬瘟热病毒感染。
A couple primers were applied to amplify the fusion region gene of canine distemper virus (CDV) fusion (F) protein gene. The templates were produced from the reverse transcription reaction that RNA were isolated from died giant pandas livers. About 320 bp fragments were amplified by polymerase chain reaction. The fragments were purified and sequence analysed. There were 281 base pairs in the fragments that excluded the 2 primers. The homologies with Beijing field strains, Harbin field strains, CDV vaccine attenuated strains, CDV Onderstepoort attenuated strains, phocine distemper virus type 2 (PDV 2) in nucleotide and amino acid were 92.9% and 98.9%, 92.5% and 98 9%, 91.5% and 94.6%, 92.9% and 98.9%, 98.2% and 100% respectively.
出处
《中国兽医学报》
CAS
CSCD
北大核心
1999年第5期448-450,共3页
Chinese Journal of Veterinary Science
