摘要
目的观察健骨颗粒含药血清对人成骨细胞增殖及芳香化酶活性的影响.方法制备空白血清及健骨颗粒含药血清,干预培养的人成骨细胞.MTT法观察对细胞增殖分化的影响,氚水法及RT-PCR分别观察健骨颗料含药血清对芳香化酶活性及表达的调节.结果当血清浓度为5%时,与空白血清组比较,含药血清组有促进人成骨细胞增殖的趋势,但差异无显著意义(P＞0.05);血清浓度为10%时,含药血清刺激细胞增殖的作用十分明显,两组比较P＜0.05.细胞干预72h后,加入[1β-3H]雄烯睾酮5.2bq,分别温育2、4、6、8h,结果发现,随着温育时间的增加,氚水计数率逐渐增高,6h活性增加明显,在含药组达高峰,8h含药组酶活性下降,空白组仍有微弱上升趋势.含药组氚水的计数率明显高于空白组.与空白对照组相比较,含药血清组芳香化酶基因mRNA表达增强.结论健骨颗粒含药血清可以促进人成骨细胞增殖,提高人成骨细胞内芳香化酶活性,在转录水平促进芳香化酶基因的表达.
Objectives To observe the effect of the serum containing Jian Gu Granules on cell proliferation, aromatase activity and expression in human osteoblastic cells. Methods The serums were divided into blank control group and Jian Gu Granules group then osteoblasts were processed with above two serums. MTT method was used to measure cell proliferation. Tritium water method was employed to measure the aromatase activity. Reverse - Transcription Polymerase Chain Reaction ( RT -PCR) method was used to determined expression of aromatase mRNA.Results When the concentration of the serum was 10% , compared with the control group, JianGu Granules group could stimulate osteoblast proliferation after 48h (P < 0.05) . Adding the serum contained JianGu Granules increased aomatase activity. Stimulating aomatase mRNA expression by the serum of Jian Gu Granules was also found. Conclusions The serum of Jian Gu Granules can induce human osteoblast cell proliferation and increase aromatase activity in a dose - dependent and time - dependent manner. It can stimulate the expression of aromatase mRNA in human osteoblastic cells at genetic transcription level.
出处
《中国骨肿瘤骨病》
2003年第1期51-53,47,共4页
Chinse Journal Of Bone Tumor And Bone Disease
基金
上海市教委资助(2000QN84)
