摘要
目的 观察环磷酰胺对IL 1α和TNF αmRNA在鼠根尖周炎组织中表达的影响 ,了解免疫抑制在根尖周炎中的作用。方法 16只SD大鼠开髓术前每周 1次腹腔注射环磷酰胺 ,共 3wk ,另 16只大鼠作对照 ;用原位杂交技术 ,测定免疫抑制鼠根尖周炎组织中IL 1α和TNF αmRNA表达 ,并对其进行半定量分析 ,与免疫正常组进行比较。结果 免疫抑制鼠根尖周组织阳性染色细胞计数 ,1～ 3wk逐渐2 0 0 2 0 6 13收稿 ,2 0 0 3 0 6 2 8修回 安徽省教育厅资助课题 ,No 2 0 0 1kj1181 上海第二医科大学附属第九人民医院 ,上海 2 0 0 0 11作者简介 :梅陵宣 ,男 ,39岁 ,医学博士 ,副教授 ,硕士生导师。Tel:0 5 5 1 5 118677 82 0 1;刘 正 ,女 ,71岁 ,教授 ,博士生导师增加 ,4wk后降低 ,并与根尖阴影面积呈正相关关系 (IL 1α :r=0 881,P <0 0 1;TNF α:r =0 85 5 ,P <0 0 1) ;各时间段IL 1α和TNF αmRNA表达量与免疫正常组无差别 ,阳性表达细胞类型亦无差别。结论 外周血白细胞减少对鼠根尖周炎的发生及局部IL 1α和TNF
AIM The purpose of this study was to investigate the effect of immunosuppression on the periapical inflammation by estimating the expression of the cytokine IL-1α and TNF-α in periapical lesion of rat. METHODS Sixteen SD rats received cycolophosphamide injection weekly before pulp exposure for three weeks, another 16 animals received no medication as control group. The expressions of IL-1α and TNF-α mRNA in periapical lesion were detected by methods of in situ hybridization,and semi-quantified analyzed compared with control group. RESULTS IL-1α and TNF-α mRNA expressions in treated group increased progressively from 1 to 3 week, and declined somewhat at 4 week. There was a statistically significant positive correlation between the area of the periapical lesion and the number of positive stained cell for IL-1α and TNF-α(IL-1α:r=0 881,P<0 01;TNF-α:r=0 855, P<0 01). There was no significant difference the number and type of staining cell in periapical lesions of two cytokines between two groups. CONCLUSION Reduction of circulating leukocytes may not significantly affect the development of periapical pathosis and the expression IL-1α and TNF-α(as key mediators) in this model.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2003年第2期209-213,共5页
Chinese Pharmacological Bulletin
基金
安徽省教育厅资助课题
No 2 0 0 1kj118
