摘要
目的 :探讨加减下瘀血汤对人肾小球系膜细胞 (HMC)表面清道夫受体A(SR -A)表达的影响。方法 :通过采用体外培养HMC ,在佛波酯 (PMA)刺激SR -A上调后 ,用加减下瘀血汤含药血清对其进行干预 ,观察加减下瘀血汤对HMC表面SR -AmRNA表达水平及SR -A与配体结合能力的影响。结果 :(1)体外培养的HMC在PMA的刺激下SR -AmRNA水平明显增高 ,摄取乙酰化低密度脂蛋白 (AC -LDL)能力增强 ,其作用随药物浓度增加而增强 (P <0 .0 5 ) ;(2 )中、高浓度的加减下瘀血汤含药血清能下调PMA诱导SR -AmRNA水平的上调 ,低浓度含药血清作用不明显。且该作用随着药物浓度的增加、作用时间的延长而增强 (P <0 .0 5 )。结论 :加减下瘀血汤治疗肾病的机理之一 ,可能是通过抑制病理状态下HMC表面SR -A的高表达。
Objective:To observe the influence of Jiajianxiayuxuetang on expressing the scavenger receptor(SR) of Human Mesangial cell(HMC)in vitro.Methods:Obtaining HMC by primary culture in vitro,using phorbol ester(PMA) up-regulation of SR-A,interfering with serum including different density of Jiajianxiayuxuetang,then observing the expressing level of SR-A mRNA and the capability SR-A integrating with its ligand on the suface of HMC. Results: (1)After stimurated by PMA SR-A mRNA up-regulated significantly and the carability of AC-LDL intake increased.The effect increased with the concentration ( P <0.05).(2)Middle and high concentration of the herbs in serun could depress the up-regulation of SR-A mRNA stimulated by PMA.And the influence strenggthened with the medicine concentratain and the affecting time( P <0.05).Conclusion:The rats' serum including Jiajianxiayuxuetang can depress the up-regulation of SR-A mRNA stimulated by PMA, and the influence strengthens with the medicine density and the affecting time. Jiajianxiayuxuetang is helpful in treating glomerulosclerosis.
出处
《中国中西医结合肾病杂志》
2003年第2期67-70,共4页
Chinese Journal of Integrated Traditional and Western Nephrology
基金
浙江省中医药管理局基金资助项目 (No .2 0 0 0Y0 8)
