摘要
目的 :研究钾通道阻断剂、一氧化氮 (NO)对继代培养大鼠肺动脉平滑肌细胞 (PASMC)钾电流 (IK)的作用。 方法 :使用全细胞膜片钳技术观察继代培养大鼠 PASMC IK 及钾通道阻断剂格列本脲 (Gli) ,四乙胺 (TEA) ,4 -氨基吡啶 (4- AP)和 NO供体 S-亚硝基乙酰青霉胺 (SNAP)对其的作用。 结果 :继代培养大鼠 PASMC较急性分离者之 IK 明显降低。 4 - AP和高浓度TEA显著降低继代培养大鼠 PASMC之 IK,SNAP显著增加继代培养大鼠 PASMC之 IK。结论 :继代培养大鼠 PASMC钾通道特性发生改变 ,但通道阻断剂和 NO仍能显著改变其活性。
Objective: To study the effect of potassium channel inhibitor and nitric oxide(NO) on the potassium current of sub cultured rat pulmonary artery smooth muscle cells(PASMC). Methods: The potassium current (I K) of sub cultured rat PASMC and the effect of potassium channel inhibitor Gli, TEA, 4 AP, and NO donor SNAP on it were observed by whole cell patch clamp techniques. Results: The I K of sub cultured rat PASMC was remarkably reduced than that of the acute dispersed ones. 4 AP and high concentration TEA remarkably reduced I K of sub cultured rat PASMC, while SNAP remarkably increased the I K. Conclusion: The character of sub cultured rat PASMC potassium channel is remarkably changed, but the potassium channel inhibitors and NO can still change its activity remarkably.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2003年第2期192-195,共4页
Academic Journal of Second Military Medical University
关键词
肺动脉
平滑肌细胞
钾通道
一氧化氮
膜片钳
pulmonary artery
smooth muscle cells
potassium channel
nitric oxide
patch clamp
