摘要
通过单因子及正交实验对一株胶质芽孢杆菌NS0 1的培养基组成 (C、N、生长因子、无机盐 )及工艺条件 (温度、起始pH、装液量 )进行了研究。结果表明比较理想的培养基配方为 (% ) :葡萄糖 1,硫酸铵 0 .15 ,酵母膏 0 .0 1,KCl0 .0 1,MgSO4 ·7H2 O 0 .0 1,NaH2 PO4 0 .0 1,CaCO30 .1。起始 pH 7.5 ,培养温度 36℃ ,装液量 5 0mL/ 2 5 0mL锥形瓶。在此条件下活菌数可达 6 .5亿 ,是传统的胶质芽孢杆菌淀粉铵培养基下活菌数的 2 6 0 %。
The media components and cultivation conditions of Bacillus mucilaginosus NS01 were investigated through single-factor and orthogonal tests.The tests show the optimum fermentation medium is consisted of glucose 10 g,(NH 4) 2SO 4 1.5 g,KCl 0.05 g,yeast extract paste 0.1 g.MgSO 4.7H 2O 0.1 g,Na 2HPO 4 0.1 g and CaCO 3 1 g every litre.The incubate condition is:temperature 36 ℃,50mL inventory/250 mL flask and pH7.5.In this condition the value of OD of the bacteria is 164% of the traditional one.
出处
《南昌大学学报(理科版)》
CAS
北大核心
2002年第3期299-302,共4页
Journal of Nanchang University(Natural Science)