摘要
目的 探讨饮用贵州茅台酒 (下称茅台酒 )对肝纤维化发生的影响及其可能的作用机制。方法 ( 1)雄性SD大鼠用茅台酒灌胃连续 5 6天后 ,处死并剖腹取肝 ,测肝组织金属硫蛋白和丙二醛含量 ;( 2 )分离鼠肝星状细胞及人肝星状细胞株做体外试验 ,观察茅台酒对肝星状细胞增殖和胶原生成的影响 ;( 3)SD大鼠茅台酒灌胃 ,连续 14周 ,取肝右叶进行病理组织学检查。结果 ( 1)茅台酒诱导大鼠肝脏金属硫蛋白含量比正常对照组增加 2 2倍 ,经茅台酒诱导处理的大鼠用CCl4中毒损伤 ,肝脂质过氧化产物丙二醛的形成较对照组明显减少 (P <0 0 5 ) ;( 2 )茅台酒呈浓度依赖性抑制肝星状细胞增殖 ,对胶原基因的表达与蛋白质分泌均有一定的抑制作用 ;( 3)茅台酒诱导高脂低蛋白饮食大鼠肝内有脂滴沉积 ,并有脂肪变性 ,肝间质仅有轻度纤维化 ,乙醇对照组大鼠肝脏呈典型肝硬化改变。结论 茅台酒诱导肝脏金属硫蛋白含量增加 ,从多环节抑制星状细胞的活化及其胶原蛋白生成可能是干预肝纤维化形成的重要机制。
Objective To explore the effect on liver and the mechanism of preventing heatic fibrosis by drinking Kweichow Moutai liquor (Maotai). Methods (1) After ingested with Maotai for 56 days consecutively, the male SD rats were decollated for detecting metallothioneins and MDA content in liver tissues; (2) Culturing rat hepatic stellate cell (HSC) and human HSC in vitro, observing the effect of Maotai on HSC′s proliferation and collagen synthesis; (3) After male SD rats were ingested with Maotai for 14 weeks consecutively, the livers were harvested for pathohistological examination. Results (1) Metallothioneins content in the liver of Maotai-induced rats increased by 22 folds, the production of hepatic lipid peroxide, MDA was significantly decreased (P<0.05) in Maotai-induced animals suffering from CCL4; (2) Maotai demonstrate obvious inhibitory effect aginst proliferation of HSC, and the inhibition was concentration-dependent. gene expression and protein secretion of collagens could also be inhibited by Maotai; (3) In control alcoholic group, typical cirrhosis of liver was shaped. In Maotai group, however, though fatty degeneration of hepatocytes and mild fibrosis of interstitium were observed, no obvious hepatic fibrosis and cirrhosis were found. Conclusion It might be an important mechanism of interfering hepatic fibrosis progressing that Maotai induces the increase of metallothioneins content in the liver, inhibits the activation HSC and the synthesis of collagen protein.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2003年第3期237-241,共5页
National Medical Journal of China
基金
贵州省科学技术重点研究课题资助项目 (黔科合计字 19992 0 15 )
