摘要
目的 :研究腹膜透析液对大鼠腹膜间皮细胞CD40表达的影响及CD40活化后与细胞间粘附分子 - 1(ICAM - 1)分泌的相关性 ,以揭示腹膜透析时腹腔局部炎症的发生机制。方法 :分离、培养大鼠腹膜间皮细胞 ,分别用IFN -γ、4 2 5 %腹透液、4 2 5 %腹透液 +IFN -γ作为刺激因子 ,通过逆转录 -多聚酶链反应 (RT -PCR)及流式细胞仪 (FACS)检测间皮细胞CD40表达 ;并通过CD40单克隆抗体 (CD40mAb)活化高表达的间皮细胞CD40 ,用FACS检测间皮细胞ICAM - 1的表达。结果 :腹膜间皮细胞结构性表达低水平CD40mRNA及蛋白。用 4 2 5 %腹透液刺激后 ,间皮细胞CD40mRNA及蛋白的表达显著高于对照组。用 4 2 5 %腹透液 +IFN -γ刺激后 ,间皮细胞表面CD40mRNA及蛋白的表达进一步增加 ,且明显高于单纯腹透液刺激组。活化CD40受体可显著增强间皮细胞ICAM - 1的表达。结论 :腹膜间皮细胞可表达CD40 ,4 2 5 %透析液及IFN -γ均能显著增加腹膜间皮细胞CD40的表达。间皮细胞CD40的上调 ,可能是腹透过程中腹腔局部炎症启动。
AIM: To investigate the effect of 4.25%peritoneal dialysis solution (PDS) on CD40 expression in rat peritoneal mesothelial cells so as to reveal the potential mechanisms by which CD40-CD40 ligand (CD40L) interaction may be involved in the inflammation of peritoneal membrane. METHODS: Rat peritoneal mesothelial cells (MC) were harvested from the peritoneal cavity and maintained under defined in vitro conditions. Expression of CD40 on MC under normal culture or stimulation with 4.25%PDS or 4.25%PDS+IFN-γ was detected by RT-PCR and FACS analyses. After activation of CD40 on MC with CD40 mAb, the expression of intercellular adhesion molecule-1 (ICAM-1) on MC was analyzed by FCAS. RESULTS: MC cultured in vitro expressed CD40 constitutively. 4.25%PDS markedly up-regulated the expression of CD40 mRNA and its protein. The expression of CD40 mRNA and its protein following stimulation with 4.25%PDS+IFN-γ was significantly higher than 4.25%PDS alone. The expression of ICAM-1 on MC was significantly increased after activation of CD40 with CD40mAb.CONCLUSIONS: MC functionally express CD40. The up-regulated CD40 expression on MC following stimulation with 4.25%PDS may play an important role in local peritoneal defense mechanisms and may be involved in the chronic inflammatory process of the peritoneum.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2003年第3期340-344,共5页
Chinese Journal of Pathophysiology
基金
卫生部重点科研基金资助项目 (No .970 40 2 2 8)
关键词
腹膜透析液
CD40
大鼠
腹膜疾病
Peritonitis
Peritoneal dialysis solution
Antigens, CD40
Rats
