摘要
为了提高降烟秆木质素粗毛栓菌(Trametes hirsuta)S13液态发酵生物量,获得菌龄整齐、特性稳定的菌丝体,采用正交设计及单因素分析技术优化了粗毛栓菌S13培养基及发酵条件。获得最佳培养基配方为:蔗糖20 g/L,玉米粉20 g/L,豆粕60g/L,K2HPO41.0 g/L,MgSO40.5 g/L,KCl 0.5 g/L;最佳发酵工艺参数为:初始pH 5.0,接种3块菌塞,500 m L三角瓶装液量80mL,180 r/min,30℃发酵5 d。在此发酵工艺条件下,粗毛栓菌S13液态发酵生物量得到显著提高,可达49.83%(V/V)。该液态发酵工艺为粗毛栓菌S13在工业上的应用奠定了理论基础和技术支持。
To improve the liquid fermentation biomass and obtain regular ages and stable characteristics of tobacco stalks lignin degrading strain Trametes hirsuta S13,the fermentation medium and cultivation conditions were optimized for T.hirsuta S13 in flasks used orthogonal and single factor analysis test.The optimum fermentation medium of T.hirsuta S13 contains sucrose 20 g/L,corn flour 20 g/L,soybean meal 60 g/L,MgSO40.5 g/L,K2HPO4 1 g/L,KCl 0.5 g/L.The optimal cultivation conditions was as following:temperature 30℃,initial pH 5.0,inoculum amount3 fungus plugs,180 r/min,broth content 80 mL/500 mL flask.Under the optimal fermentation medium and conditions,the biomass of T.hirsuta S13 was 49.83%(V/V).The liquid fermentation technology can provides experimental basis for industrial application.
作者
杨亮
宋丹丹
李琼
时伟
陈守文
马昕
YANG Liang;SONG DANDan;LI Qiong;SHI Wei;CHEN Shouwen;MA Xin(Department of Brewing Engineering,Maotai College,Renhuai 564501;College of Life Sciences,Hubei Universi?ty,Wuhan 430062,China)
出处
《东北农业科学》
北大核心
2019年第4期76-80,共5页
Journal of Northeast Agricultural Sciences
基金
贵州省教育厅科技拔尖人才支持项目(黔教合KY字[2018]082)
贵州省教育厅青年科技人才成长项目(黔教合KY字[2018]442)
关键词
粗毛栓菌
木质素降解菌
液态发酵
烟秆
Trametes hirsuta
Lignin degradation microorganisms
Liquid fermentation
Tobacco stalk
