摘要
利用异丙肾上腺素诱发小鼠心肌缺血模型,考察不同配比的丹参-檀香提取物对血清和心脏组织中主要心肌缺血生物标志物的活力、含量、相对表达量以及病灶组织变化的影响,并初步探讨了其作用机制。结果表明:丹参檀香不同配比提取物均能降低异丙肾上腺素诱导的心肌缺血损伤程度,显著改善心肌缺血引起的心脏组织结构病变;所有给药组和阳性对照组均能不同程度地改善血清肌酸磷酸激酶(CK)、乳酸脱氢酶(LDH)和心脏组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)指标的变化,对CK和LDH影响最为显著的是给药组1(丹参高剂量+檀香高剂量)和给药组2(丹参高剂量+檀香低剂量);对SOD、GSH-Px和MDA影响最为显著的是给药组1(丹参高剂量+檀香高剂量)和给药组3(丹参低剂量+檀香高剂量); qRT-PCR结果表明,丹参-檀香提取物预防心肌细胞坏死的机理与诱导Bcl-2基因表达和抑制Bax基因表达有关,且该作用与药对丹参中成分的含量有明显的剂量依赖性。综上,丹参-檀香药具有良好的抗心肌缺血损伤的作用,其中丹参在预防心肌细胞凋亡方面的影响更为明显,檀香的抗氧化作用较强,檀香与丹参合用会产生一定的协同作用,可增强治疗效果。
The effects of Salvia miltiorrhiza and Santalum album extracts(SSE)on isoprenaline(ISO)induced mice model of myocardial ischemia(MI)were investigated by measuring activity,content and 2-ΔΔCtof several typical biomarkers related to MI,with observation to the pathological changes of injury cardiac tissues.It turned out that all of the tested groups of SSE were discovered to ameliorate heart injury and pathological tissues of mice caused by myocardial ischemia.SSE groups and positive control were both proved to be effective against either CK and LDH in serum or SOD,GSH-Px and MDA in cardiac tissue to different extents.Specifically,SSE group 1(high-dose Salvia miltiorrhiza+high-dose Santalum album)and group 2(high-dose Salvia miltiorrhiza+low-dose Santalum album)had the most significant effects on CK and LDH as well as SSE group 1 and group 3(low-dose Salvia miltiorrhiza+high-dose Santalum album)on SOD,GSH-Px and MDA.As the result of qRT-PCR,protection mechanism of SSE on MI injury was in close relation to up-regulation of Bcl-2 and down-regulation of Bax.And this mechanism was dose-dependent to the content of Salvia miltiorrhiza in SSE.In conclusion,SSE appeared to be good anti-myocardial ischemia combinations.Moreover,Salvia miltiorrhiza mainly functioned on the prevention of cardiomyocyte from apoptosis,with comparison to Santalum album which played a more important role in anti-oxidant aspects.Salvia miltiorrhiza and Santalum album could exert synergistic effects on the treatment of myocardial ischemia.
作者
何天竺
辛宇
宋岩
王伟楠
隋殿军
HE Tianzhu;XIN Yu;SONG Yan;WANG Weinan;SUI Dianjun(College of Traditional Chinese Medicine,Changchun University of Chinese Medicine,Changchun 130117,China)
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2019年第2期192-198,共7页
Journal of Jilin Agricultural University
基金
吉林重大项目前期工作课题[吉发改投资(2015)1009号]
关键词
丹参
檀香
异丙肾上腺素
心肌缺血
Salvia miltiorrhiza
Santalum album
isoprenaline
myocardial ischemia
