摘要
目的 :探讨阳离子脂质体Lipofectin对bcl xL反义寡核苷酸进入CNE 2Z细胞的影响。方法 :采用阳离子脂质体Lipofectin介导bcl xL反义寡核苷酸转染CNE 2Z细胞 ,应用MTT法观察Lipofectin介导前后对CNE 2Z增殖的影响 ;RT PCR检测处理前后bcl xLmRNA表达水平。结果 :Lipofectin介导bcl xl反义核酸可提高反义核酸对细胞的增殖抑制率 ;Lipofectin介导bcl xL反义核酸可增强反义核酸下调bcl xLmRNA表达水平的能力。 结论 :Lipofectin可促进反义核酸转染进入细胞。
Objective:To examine the effect of lipofectin(Lip)on the entrance of bcl xL antisense oligodeoxynuceotide(ASODN)into nasopharyngeal carcinoma(NPC)cell line.Methods:After NPC cell line CNE 2Z was transfected by lipofectin mediated bcl xL ASODN,CNE 2Z cell proliferation and bcl xL mRNA expression were assessed by MTT assay and RT PCR,respectively.Results:Compared with application of ASDON alone,the inhibition of cell proliferation and down regulation of bcl xL mRNA expression were much obvious after treatment with ASDON/Lip complex.Conclusion:Lipofetin enhances cellular uptake of bcl xL ASODN in CNE 2Z cells.
出处
《广东医学院学报》
2003年第1期1-4,共4页
Journal of Guangdong Medical College
基金
广东省重点基金资助项目 (ZKM 0 4 50 5S)
