摘要
目的探讨小鼠骨髓间充质干细胞来源的外泌体(bone marrow mesenchymal stem cell-derived exosomes,BMSCs-exo)对紫外线辐射后的鼠真皮成纤维细胞(Fibroblasts,FBs)的保护作用。方法用流式细胞术鉴定小鼠骨髓间充质干细胞(BMSCs),提取并鉴定其上清液来源的外泌体(exosome)。用紫外线照射FBs建立细胞光老化模型,采用不同浓度的BMSCs-exo处理照射后的FBs。采用β-半乳糖苷酶染色法进行FBs衰老检测。通过Western blot检测处理后FBs的I型胶原蛋白,基质金属蛋白酶-1(matrix metalloproteinase-1,MMP-1)和弹性蛋白的蛋白质水平。结果β-半乳糖苷酶染色法显示BMSCs-exo浓度越高细胞衰老数目越少。Western blot检测显示BMSCs-exo浓度越高MMP-1表达越低,I型胶原蛋白和弹性蛋白表达越高。结论 BMSCsexo可以缓解紫外线诱导的鼠真皮成纤维细胞光老化进展。
Objective To investigate the protective effect of mouse bone marrow mesenchymal stem cellderived exosomes(BMSCs-exo)on murine dermal fibroblasts(FBs)after UV irradiation.Methods First,mouse bone marrow mesenchymal stem cells(BMSCs)were identified by flow cytometry,and exosomes derived from the supernatant were extracted and identified.Second,UV-irradiated FBs were used to establish a photoaging model of cells,and FBs after irradiation were treated with different concentrations of BMSCs-exo.FBs senescence detection was performed byβ-galactosidase staining.Finally,Western blot was used to detect the protein levels of type I collagen,matrix metalloproteinase-1(MMP-1)and elastin in FBs after treatment.Resultsβ-galactosidase staining showed that the higher the concentration of BMSCs-exo,the smaller the number of cell senescence.Western blot assay showed that the higher the concentration of BMSCs-exo,the lower the expression of MMP-1,and the higher the expression of type I collagen and elastin.Conclusion BMSCs-exo can alleviate the progress of UV-induced photoaging of dermal fibroblasts.
作者
赵翡
朱华
马海英
卢晓梅
马玲
于艳秋
ZHAO Fei;ZHU Hua;MA Hai-ying;LU Xiao-mei;MA Ling;YU Yan-qiu(Department of Pathophysiology,School of Basic Medicine,China Medical University;Shenyang cell therapy engineering technology research and Development Center Limited company,Shenyang 110122,China)
出处
《解剖科学进展》
2019年第5期548-551,共4页
Progress of Anatomical Sciences
基金
国家自然科学基金(2600015005)
