摘要
目的探讨构建2型糖尿病(T2DM)下肢血管病变球囊扩张术(TPA)后再狭窄的SD大鼠模型的方法。方法 30只T2DM大鼠分为以下3组:动脉粥样硬化组(AS)、再狭窄组(RS)和对照组,HE染色观变血管形态及结构变化。α-平滑肌肌动蛋白(α-SMA)免疫组织化学染色对斑块组成成分进行检测。结果 RS组可见明显的内膜增生,形成再狭窄斑块。构成RS斑块的细胞主要是平滑肌细胞(SMCs)。结论采用球囊拉伤联合球囊扩张术成功构建了糖尿病大鼠下肢血管病变PTA后RS动物模型。
Objective To establish an animal model of restenosis in diabetic lower extremity arterial disease rat after percutaneous transluminal angioplasty(PTA).Methods We divided diabetic rats(n=30) into three groups:atherosclerosis group(AS),restenosis group(RS) and control group.We examined the artery by hematoxylin and eosin(HE),and detected the plaque components by α smooth muscle actin(α-SMA) immunohistochemical staining.Results Artery restenosis rat models were successfully developed.The smooth muscle cells(SMCs) were the key components of the RS plaque.Conclusion An experimental animal model of restenosis in diabetic lower extremity arterial disease rat after PTA is set up by the steps described in this study.
作者
邹志伟
张倩
董建军
ZOU Zhi-wei;ZHANG Qian;DONG Jian-jun(Department of Endocrinology,Qilu Hospital of Shandong University,Ji’nan 250012,China)
出处
《解剖学报》
CAS
CSCD
北大核心
2019年第1期128-131,共4页
Acta Anatomica Sinica
基金
国家自然科学基金(81670757)
关键词
糖尿病
再狭窄
血管平滑肌
球囊扩张术
HE染色
免疫组织化学法
大鼠
Diabetes
Restenosis
Vascular smooth muscle cell
Percutaneous transluminal angioplasty
HE staining
Immunohistochemistry
Rat
