摘要
研究了茎尖大小、接种方式、培养基成分、试材基因型对甜樱桃品种茎尖培养的影响。 1年生成熟枝条上茎尖成苗率为 8.3%～ 2 3.7% ,嫩梢上的茎尖成苗率为 2 7.3%～ 37.5 %。利用RT PCR技术对部分甜樱桃试管苗进行了早期病毒鉴定 ,筛选出一些不带李坏死环斑病毒 (PNRSV)的甜樱桃试管苗 ,并证明甜樱桃试管苗微茎尖培养不能有效脱除PNRSV。
The effects of size of inoculated shoot tip, method of inoculation, medium and genotype on shoot tip culture of sweet cherry ( Prunus avium L.) cultivars were studied. The rate of shoots forming from tips of one year old twigs was low, 8.3% to 23.7%, while the rate of shoots forming from tips of actively growing shoots was higher, 27.3% to 37.5%. Prunus necrotic ringspot virus (PNRSV) was detected early in some of sweet cherry plantlets in vitro with RT PCR technique. Plants free of PNRSV were found. It was proved that PNRSV could not be eliminated effectively from sweet cherry plantlets in vitro by culture of minor shoot tip.
出处
《园艺学报》
CAS
CSCD
北大核心
2003年第1期87-89,共3页
Acta Horticulturae Sinica
