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诱导黄瓜直接器官发生主要影响因素的研究 被引量:51

Main Factors Influencing the Frequency of Direct Organogenesis of Cucumber in Vitro
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摘要 对诱导黄瓜直接器官发生的主要因素进行了探讨。结果表明 :外植体类型、基因型及植物生长调节剂对再生频率均有显著影响。子叶节是最佳的外植体类型 ;‘长春密刺’及‘龙杂黄七号’再生频率较高 ,分别达到 93.2 %和 92 .5 % ,前者每块外植体上的平均芽数 (13.2 )极显著高于后者 (3.1) ;6 BA单独存在即可诱导产生丛生芽 ,0 . The main factors influencing the frequency of direct organogenesis of cucumber( Cucumis sativus L.)were discussed.The results showed that the types of explants,genotypes and plant growth regulator had significantly effects on regenerating frequency.The optimum explant is cotyledonary nodes.Genotypes'Changchun mici'and'Longzahuang 7'had higher frequency of regeneration which were 93.2% and 92.1% respectively. But the former differentiated much more buds(13.2 per explant)than that of the latter(3.1 per explant). 6 BA can effectively induced buds and the optimum concentration is 0.5mg/L.
出处 《园艺学报》 CAS CSCD 北大核心 2003年第1期101-103,共3页 Acta Horticulturae Sinica
关键词 影响因素 黄瓜 再生体系 直接器官发生 芽分化 外植体 植物生长调节剂 基因型 Cucumber High frequency regenerated system Direct organogenesis Differentiation rate of buds
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  • 1赵军良,马蓉丽,李昌华.黄瓜子叶组织培养再生植株[J].山西农业科学,1996,24(1):39-41. 被引量:31
  • 2[2]Ladyman J A R,Girard B.Cucumber somatic embryo development on various gelling agents and carbohydrate sources.Hortsci.,1992,27:164~165
  • 3[3]Colijin-Hooymans C M,Hakkert J C.Competence for regeneration of cucumber cotyledons restricted to specific developmental stages.Plant Cell,Tissue and Organ Culture,1994,39:211~217

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