摘要
目的 :研究雌激素受体 β亚型 (ERβ)在大鼠睾丸发育过程中的作用 .方法 :采用免疫组织化学染色和免疫荧光组织化学染色方法 ,观察在出生后 3,7,16及 2 8d的大鼠睾丸和成年大鼠睾丸中ERβ的表达和分布情况 .结果 :在出生后 3,7d的大鼠睾丸中ERβ主要分布于睾丸间质细胞 ,在出生后 16 ,2 8d分布于睾丸间质细胞胞质 ,大鼠睾丸中ERβ的表达随出生天数增加而呈逐渐上升的趋势 ,成年大鼠睾丸间质细胞中ERβ分布较幼年大鼠密度增加 .结论 :ERβ可能作为一种特异性受体在大鼠睾丸发育过程中影响睾丸间质细胞雄性激素的分泌 ;进而调节生精过程和精子发育成熟 .
AIM: To investigate the expression and localization of estrogen receptor sub type β (ERβ) in rat testis during postnatal developing. METHODS: In this study, whole testes were collected from male SD rats aged 3, 7, 16 and 28 d as well as adult rats. By immunohistochemistry and fluo immunohistochemistry, expression and distribution of ERβ were examined during rat testicular developing. RESULTS: In ages of 3, 7, 16, 28 d and adult rat testes, the immunostaining of ERβ was present in Leydig cells of testes and the positive substance was located in the cytoplasm. The number of ERβ positive cells was increasing gradually with the rat development. CONCLUSION: ERβ may affect excreting of androgen in Leydig cells as special receptor, which provides direct evidence for molecular mechanism of ERβ action during postnatal development and spermatogenesis of rats.
出处
《第四军医大学学报》
北大核心
2003年第4期296-298,共3页
Journal of the Fourth Military Medical University
