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利用三种分子标记研究缘毛类纤毛虫的系统发育地位 被引量:4

Phylogenetic Position of Peritrichs Based on Tree Kinds of Molecular Markers
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摘要 为了探讨缘毛类纤毛虫的系统发育地位 ,利用RAPD方法得到了 9种缘毛类纤毛虫、 1种四膜虫和1种喇叭虫的 3个随机引物的电泳带谱 ;测定了 7种缘毛类纤毛虫rRNA基因中的间隔区 1(ITS1)和小亚基核糖体核糖核酸 (SSrRNA)基因序列 ,并构建了相应的系统树。在比较和分析RAPD、ITS1和SSrRNA基因序列在缘毛类纤毛虫系统发育研究中的适用范围的基础上 ,以SSrRNA基因序列为分子标记研究了缘毛类纤毛虫系统发育地位 ,结果表明 :①缘毛亚纲是单系的 ,作为寡膜纲中一个亚纲的分类地位是合理的 ;②缘毛类纤毛虫可能是寡膜纲中较高等的一个类群。 Using the method of random amplified polymorphic DNA (RAPD)and three primers,we got the sequences of the first internal transcribed spacer region (ITS1) and a small subunit of rRNA (SSrRNA),and then constructed phylogenetic trees of some genera/species in peritrichs.A comparison of the tree structures showed that SSrRNA was more reliable marker for analyzing the phylogenetic position of peritrichs within Ciliophora.The phylogenetic tree based on the SSrDNA sequence strongly supported the monophyly of the subclass Peritrichia,or its subclass rank is reasonable.In addition,there are some evidences showing that peritrichs are a higher group in Oligohymenophoreans.
出处 《Zoological Research》 CAS CSCD 北大核心 2003年第1期1-10,共10页 动物学研究(英文)
基金 国家自然科学基金资助项目 (3 973 0 0 70 ) 东湖湖泊生态系统试验站开放基金资助项目 (2 0 0 10 4)
关键词 分子标记 缘毛类纤毛虫 系统发育地位 随机扩增多态DNA 间隔区1 小亚基核糖体核糖核酸 Random amplified polymorphic DNA First internal transcribed spacer region Small subunit rRNA Phylogeny
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