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长春花黄化植原体(PY)株系的检测与鉴定 被引量：15

Detection and Identification of Phytoplasma Strain Associated with Periwinkle Yellows
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摘要 A 1.2kb and 1.3kb DNA fragments were amplified in DNA samples extracted from periwinkle exhibiting yellows naturally. The amplified fragments were cloned, sequenced and compared with other phytoplasma strains. The results showed that the 16S rDNA and ribosomal protein gene of periwinkle yellows phytoplasma consist of 1239bp and 1244bp, respectively. They all shared high homology (higher than 97.5%) with phytoplasma strains of 16S rI group. Thus, the result made it clear that the phytoplasma strain of periwinkle yellows (PY) is one of the members of phytoplasma 16SrI group. A 1.2kb and 1.3kb DNA fragments were amplified in DNA samples extracted from periwinkle exhibiting yellows naturally. The amplified fragments were cloned, sequenced and compared with other phytoplasma strains. The results showed that the 16S rDNA and ribosomal protein gene of periwinkle yellows phytoplasma consist of 1239bp and 1244bp, respectively. They all shared high homology (higher than 97.5%) with phytoplasma strains of 16S rI group. Thus, the result made it clear that the phytoplasma strain of periwinkle yellows (PY) is one of the members of phytoplasma 16SrI group.
作者 蔡红 孔宝华 陈海如
机构地区 云南农业大学云南省植物病理重点实验室
出处 《微生物学报》 CAS CSCD 北大核心 2003年第1期116-119,共4页 Acta Microbiologica Sinica
基金 云南省自然科学基金 ( 2 0 0 0C0 0 1 4Q) 云南省省院省校科技合作基金 ( 98YQ0 0 9)资助项目
关键词 长春花 黄化植原体 检测 鉴定 核糖体蛋白基因 Periwinkle, Yellows phytoplasma, 16S rRNA gene, Ribosomal protein gene
分类号 S432.4 [农业科学—植物病理学]
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参考文献11

  • 1Lee I M, Gundersen-Rindal D E, Davis R E, et al. Revised classification scheme of phytoplasmas based on RFLP analyses of 16S rRNA and ribosomal protein gene sequences. International Journal of Systematic Bacteriology, 1998, 48: 1153～1169.
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微生物学报
2003年 第1期

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