摘要
目的 观察G蛋白α亚基mRNA在不同甲状腺疾病的表达。方法 收集手术切除的甲状腺组织包括Graves病 5例、结节性甲状腺肿 7例、正常功能甲状腺腺瘤 6例和甲状腺乳头状癌 5例 ,以腺瘤周围组织作为正常甲状腺组织对照 ,观察刺激性G蛋白 (Gs)和抑制性 (Gi)α亚基的表达。结果 (1 )GsαmRNA的表达 :甲状腺乳头状癌 (1 67± 0 2 5)较正常甲状腺组织 (1 1 0± 0 1 4 )和结节性甲状腺肿 (0 96± 0 31 )明显增高 (P <0 0 5和P <0 0 1 ) ,Graves病 (1 47± 0 1 1 )和正常功能甲状腺腺瘤 (1 36± 0 2 8)均显著高于结节性甲状腺肿 (P <0 0 5) ,但与正常甲状腺组织表达水平相似 ;(2 )Giα 2mRNA的表达 :Graves病显著低于结节性甲状腺肿 (0 68± 0 2 6比 1 1 5± 0 35 ,P <0 0 5) ;(3)Giα 1和Giα 3mRNA在不同甲状腺疾病的表达相似。结论 Gs蛋白α亚基的高表达可能在甲状腺乳头状癌的发病中起重要作用 。
Objective To investigate the expression of G proteins α subunit mRNA in different thyroid diseases Methods Twenty three thyroid specimens were obtained during surgery, 5 from patients with Graves′ disease (GD), 7 from patients with multinodular goiter (MNG), 6 from patients with eufunctioning thyroid adenoma (EFTA) and 5 from patients with thyroid papillary cancer (TPC) The expression of stimulating and inhibiting G proteins α subunit mRNA were determined by reverse transcription polymerase chain reaction (RT PCR) Results (1) The expression of Gsα mRNA in TPCs(1 67±0 25)was significantly higher than that in normal thyroids (1 10±0 14) and MNGs(0 96±0 31), P <0 05 and P <0 01 The expression in GDs (1 47± 0 11)and EFTAs (1 36±0 28)was significantly higher than that in MNGs( P <0 05), but comparable to that in normal thyroids (2)The expression of Giα 2 mRNA in GDs (0 68±0 26)was lower than that in MNGs (1 15± 0 35), P <0 05, but there was no difference in the expression of Giα 1 and Giα 3 mRNA among different thyroid diseases Conclusion The results indicated that Gsα could play an important role in the pathogenesis of thyroid papillary cancer and G proteins had different expression in different thyroid diseases
出处
《中华内科杂志》
CAS
CSCD
北大核心
2003年第2期103-105,共3页
Chinese Journal of Internal Medicine
基金
国家自然科学基金资助项目 ( 394 70 339)
