摘要
目的 :为通痹止痛胶囊的提取工艺优选最佳条件。方法 :以乌头碱、总生物碱和干膏得率为指标 ,以TLCS和可见紫外分光光度法为测定方法 ,采用均匀设计法优选其半仿生提取法 (简称SBE法 )的条件。结果 :3煎用水的pH值依次为 2 0 ,7 0 ,10 0 ;加水 18倍量 ,煎煮 3h为最佳提取条件。结论 :用均匀设计法优选SBE法工艺条件 。
Objective:To optimize the extraction conditions in the modification of the formula of TongBiZhiTong Capsule. Mothod:The semi-bionic extraction(SBE)conditions were optimized through homogeneous design while aconitine, total alkaloids and dry extract were adopted as markers. Results:pH in the first extraction was adjusted to 2 0 by the use of HCl solution. And then pH in second and third extraction adjusted to 7 0 and 10 0. The amount of water added is 18 times. The boiling time is 3 hours respectively. Conclusion: Optimizing the exaction conditions of SBE through homogeneous design is easy to practice.
出处
《云南中医学院学报》
2003年第1期1-5,14,共6页
Journal of Yunnan University of Traditional Chinese Medicine
基金
云南省自然科学基金资助项目 (No :2 0 0 113 )
