摘要
目的 探讨鼻咽部淋巴瘤blu基因启动子的高度甲基化在肿瘤发生中的作用及在分子病理诊断中的应用价值。方法 选用 2 0例鼻咽部NK T细胞淋巴瘤 (包括NK细胞淋巴瘤、NK样T细胞淋巴瘤及外周T细胞淋巴瘤 )石蜡包埋组织 ,用甲基化特异性PCR(methylationspecificPCR ,MSP)方法检测。结果 blu基因在鼻咽部NK T细胞淋巴瘤中高度甲基化 ,出现率为 30 % (2 0例中 6例 )。其中在 15例NK细胞淋巴瘤中 4例存在blu的高度甲基化 ;2例NK样T细胞淋巴瘤中 1例有高度甲基化 ;3例外周T细胞淋巴瘤 (非特殊型 )中 1例有blu高度甲基化状态。结论 blu基因启动子的CpG岛在鼻咽部NK T细胞淋巴瘤中高度甲基化现象 ,表明此抑癌基因被失活 ,提示blu基因的高度甲基化可能与淋巴瘤的发生有关。证实MSP方法在石蜡组织标本中可以检测基因甲基化。blu基因的高度甲基化也可能为鼻咽部NK T细胞淋巴瘤提供新的肿瘤分子病理诊断标记物。
Objective To investigate the hypermethylation status of blu gene promoter in nasopharyngeal NK/T cell lymphoma and its role in the tumorigenesis and molecular diagnosis of this lymphoma. Methods Twenty cases of paraffin embedded nasopharyngeal NK/T cell lymphomas tissues were studied by using methylation specific PCR (MSP). Results Hypermethylation of blu gene promoter is detected in 6 of 20 (30%) nasopharyngeal NK/T cell lymphoma. The 6 positive cases were 4 in 15 nasopharyngeal NK cell lymphomas, 1 of 2 NK like T cell lymphoma and 1 in 3 peripheral T cell lymphomas(unspecified type). Conclusion Hypermethylation of blu gene promoter in nasopharyngeal NK/T cell lymphoma indicated the inactivation of blu gene and its possible role in the tumorigenesis of this lymphoma. blu gene methylation could be detected in paraffin embedded tissue and used as a new molecular marker for the diagnosis of this lymphoma.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2003年第3期138-140,共3页
Chinese Journal of Hematology
