摘要
研究了十二烷基硫酸钠(SDS)对高压静电场对酶作用的影响。结果表明:在强度为3×103V/cm的电场作用下,十二烷基硫酸钠使过氧化氢酶对高压静电场的作用变得更加敏感。低浓度的十二烷基硫酸钠中,过氧化氢酶在高压静电场的作用下活力升高;0.04mmol/L～0.12mmol/L的十二烷基硫酸钠中,酶活力在高压静电场作用下先升高后下降;高于0.40mmol/L,则酶活力单调下降。十二烷基硫酸钠对高压静电场中的过氧化氢酶具有一定的保护作用。
The effect of SDS on inte raction of high voltage electrostatic field(HVEF) of catalase was studied. The r esults indicated that: SDS made that catalase was more sensitive in high voltage electrostatic field than C. K. At 3×10~3v／cm. when the co ncentration of SDS was 0.02mmol／L, the a ctivity of catalase increased greatly in high voltage electrostatic field. If th e concentration of SDS was between 0.04m mol／L and 0.12mmol／L, the activity of ca talase increased firstly and then decrea sed. When the concentration was between 0.20mmol／L and 0.60mmol／L, the activity of catalase in high voltage electrostati c field decreased lonely. SDS could some what protect catalase from high voltage electrostatic. The mechanism was probabl y that SDS could form an opposite electro static field when HVEF is on solution of SDS and catalase, and the electronic fi eld strength reduced.
出处
《激光生物学报》
CAS
CSCD
2003年第1期18-21,共4页
Acta Laser Biology Sinica
