摘要
沙漠植物由于含有多糖、多酚、单宁等次生物质,要提取出质量高、纯度好的DNA比较困难。本研究以刚毛柽柳(Tamarix hispida)为材料,用高盐低pH法成功地提取出了基因组DNA,所得到的DNA片段均大于23kb,DNA得率为225μg/g干重,A_(260)/A_(280)的比值在1.7～1.9之间,说明高盐低pH法对于含多糖、酚类、单宁等化学物质较多的沙漠植物是行之有效的。研究了RAPD(随机扩增DNA多态性)技术的影响因素,摸索出适合刚毛柽柳的反应体系与扩增条件,可用于刚毛柽柳居群内、居群间遗传多样性及柽柳科其它属种分子生物学的研究。
The genomic DNA extraction from plants in desert with high quality and purity is difficult because the content of phenolic compound,polysaccharides and other secondary metabolites is high. A low pH extraction medium with high salts,which was successfully used to obtain the genomic DNA from Tamarix hispida. The DNA yields were about 225μg/g DW,the length of the DNA fragment was more than 23 kb and the A260/A280 values of isolated DNA were between 1. 7-1. 9. Influencing factors affecting the result of RAPD were tested and an optimal reaction system was established. This study provides a technology for the study on genetic diversity of Tamarix hispida in population and other species of Tamarix L.
出处
《西北植物学报》
CAS
CSCD
2003年第2期253-256,共4页
Acta Botanica Boreali-Occidentalia Sinica
基金
中国科学院"西部之光"项目(992003)
中国科学院"生物区系特别支持费"项目
