摘要
A plasmid(DNA vaccine)pSC-IL12,coexpressing hepatitis B virus(HBV)envelope-core fusion antigen and murine IL-12,was constructed.After the plasmid was transfected into COS7 cells,HBsAg and HBcAg were detected in cytoplasm,murine IL-12 was detected in supernatant.BALB/c mice were vaccinated intramuscularly with this construct or HBV envelope-core fusion antigen DNA vaccine pSC.Following twice immunizations,humoral and cellular immune responses of the mice were detected.Results showed that pSC-IL12 induced weaker antibody but stronger T lymphocyte proliferative response and cytotoxic T lymphocyte response than pSC did,which indicated that HBV fusion antigen and IL-12 coexpressing DNA vaccine may be more useful for therapy of hepatitis B.
A plasmid(DNA vaccine)pSC-IL12,coexpressing hepatitis B virus(HBV)envelope-core fusion antigen and murine IL-12,was constructed.After the plasmid was transfected into COS7 cells,HBsAg and HBcAg were detected in cytoplasm,murine IL-12 was detected in supernatant.BALB/c mice were vaccinated intramuscularly with this construct or HBV envelope-core fusion antigen DNA vaccine pSC.Following twice immunizations,humoral and cellular immune responses of the mice were detected.Results showed that pSC-IL12 induced weaker antibody but stronger T lymphocyte proliferative response and cytotoxic T lymphocyte response than pSC did,which indicated that HBV fusion antigen and IL-12 coexpressing DNA vaccine may be more useful for therapy of hepatitis B.
出处
《病毒学报》
CAS
CSCD
北大核心
2003年第1期83-85,共3页
Chinese Journal of Virology
基金
上海科技发展基金 ( 0 0 43192 0 6 )
国家自然科学基金( 39980 0 38)
