摘要
目的 建立反相高效液相色谱法测定烧伤Ⅱ号酊中盐酸小檗碱的含量。方法 固定相 :RP C18柱 ,流动相 :乙腈 醋酸乙酯 甲醇 0 .0 5mol·ml-1KH2 PO4溶液 (35∶10∶10∶6 5 ,加 1%三乙胺 ,磷酸调pH至 3.5 ) ,检测波长 :2 70nm。结果 盐酸小檗碱在0 .0 3～ 0 .10mg·ml-1浓度范围内 ,线性关系良好 ,r =0 .9996。日内及日间RSD分别为 1.5 5 %、2 .86 % (n =12 )和 1.78%、2 .6 5 % (n =4 ) ,加样回收率为 99.89% (RSD =1.18% )。结论 本法结果准确 ,灵敏度高 ,重现性好 ,可用于烧伤Ⅱ号酊剂中盐酸小檗碱的质量控制。
Aim To establish an HPLC method for determination of berberine hydrochloride in Shaoshang Ⅱ tincture.Methods Column C 18 ODS was used.The mobile phase consisted of acetonitrile ethyl acetate methanol 0.05mol·L -1 KH 2PO 4 (35∶10∶10∶65,1% triethylamine,adjust pH to 3.5 with phosphate acid).Detective wavelength was 270nm.Results The good linearity was observed for the concentration of 0.03～0.100mg·ml -1 ,r=0.999 6.The RSD of intra day was 1.55%～2.86%,n=12 and the RSD of inter day was 1.78%～2.65%,n=4.The average recovery was 99.89%(RSD=1.18%).Conclusion The method was accurate,sensitive for the determination of berbreine hydrochloride in ShaoshangⅡtincture.
出处
《解放军药学学报》
CAS
2003年第1期67-69,共3页
Pharmaceutical Journal of Chinese People's Liberation Army
