摘要
目的:探讨汉防己甲素(Tet)对急性脊髓损伤(ASCI)的保护作用及作用机制。方法:81只大鼠随机分为三组:生理盐水对照组(NS组)、汉防己甲素治疗组(Tet组)和甲基强松龙治疗组(MP组),每组27只。用加速型Allen's打击法制成脊髓急性损伤模型,监测大鼠ASCI后及用药后的血压变化,测定损伤区脊髓Ca2+、MDA含量;采用氢清除法测定脊髓伤区血流量(SCBF)的变化;连续观测6周ASCI后运动功能评分情况;ASCI后4h、8h、6周取伤区标本进行组织病理检查。结果:ASCI后10s各组动物的MABP显著升高,1min内迅速降至正常水平,之后Tet组舒张压、平均动脉压明显降低(P<0.05),而收缩压降低不明显(P>0.05);ASCI后SCBF下降,但Tet组和MP组的SCBF较NS组高(P<0.05);损伤区Ca2+及MDA含量Tet组和MP组均较NS组低,神经功能评分均较NS组高。结论:Tet能改善微循环,防止SCBF的减少;抗脂质过氧化损伤,减少脂质过氧化物MDA的生成;减轻Ca2+的局部积聚,防止钙超载,阻断继发性损伤的链式反应,减轻组织继发性损伤。对实验性急性脊髓损伤有保护作用。
Objective:To study the protective effect and mechanisms of tetrandrine on spinal cord injury of rats.Method:Mean arterial blood pressure(MABP),diastolic pressure(DP) and systolic pressure were recorded with physical machine.Concentration of calcium and MDA was assayed.Spinal cord blood flow(SCBF),motor function of hindlimbs(Wrathall′s score) and pathological changes of spinal cord was observed.Result:It was found that Tet can decrease MABP and DP siginificantly and SP slightly,prevent regionly SCBF form decreasing,reduce the regional accumulation of calcium and the production of MDA,facilitate the recovery of neural function,relieve the pathologic injuries of spinal cord.The effects were similar to that of MP.Conclusion:The results of present study suggested that tetrandrine possesses a protective effect on contused spinal cord in rats,in which the mechanisms include its ability to ameliorate local microcirculation of injuries spinal cord,to attenuate the injury of peroxidation,to reduce the calcium overload.
出处
《中国脊柱脊髓杂志》
CAS
CSCD
2003年第3期160-163,T001,共5页
Chinese Journal of Spine and Spinal Cord
关键词
汉防己甲素
脊髓损伤
治疗
机制
大鼠
Spinal cord injury
Tetrandrine
Therapy
Mechanism
Rat
