摘要
目的 观察原钒酸钠对Ⅱ型糖尿病大鼠的降糖作用。方法 用高脂饲料灌胃正常大鼠 ,引起肥胖 ,测定血中游离脂肪酸浓度。同时应用正糖钳技术检测胰岛素抗性 ,对产生胰岛素抵抗的大鼠腹腔注射小剂量链脲菌素 (5 5mg·kg-1) ,然后筛选空腹血糖值大于 11 1mmol·L-1大鼠为糖尿病模型组。连续灌胃原钒酸钠 7d后 ,测定空腹血糖值。结果 ①大鼠喂食高脂饲料后 ,正糖钳实验中维持血糖稳态所需胰岛素量增多 ,为 (0 5 4± 0 0 2 )U·min-1,高于正常组 (P <0 0 1) ;同时血中游离脂肪酸浓度增加 ,从正常 (0 4 6 9±0 0 4 7)mmol·L-1至 (1 5 32± 0 2 91)mmol·L-1(P <0 0 1) ;②原钒酸钠对正常大鼠的血糖值无影响 ,而对Ⅱ型糖尿病大鼠的空腹血糖值及糖耐量曲线下面积有降低作用 (P <0 0 5 )。结论 实验结果证明了原钒酸钠可以明显降低Ⅱ型糖尿病大鼠的空腹血糖值 。
AIM To observe the anti hyperglycemic effect of sodium orthovanadate on type 2 diabetes rats. METHODS Rats were fed by highly fatted food to grow into obesity. Then values of plasma free fatty acid (FFA) were tested. Glucose disposal was measured with the hyperinsulinemic euglycemic clamp technique to assess insulin resistance in vivo. Small dose of streptozocin was injected ip with insulin resistance. Those with plasma glucose over 11 1 mmol·L -1 were assigned to type 2 diabetes model group, continually fed with sodium orthovanadate for 3 days and assayed the values of plasma glucose in fasting state. RESULTS ①Rats fed with fatted food needed more insulin to maintain normal plasma glucose, ie (0 54±0 02) U·min -1 , higher than that of normal control ( P <0 01), and the levels of FFA increased from (0 47±0 05) mmol·L -1 to (1 53±0 29) mmol·L -1 ( P <0 01); ②sodium orthovanadate had no effect on values of plasma glucose in normal controls, but it could decreased that of type 2 diabetes rats in fasting state ( P <0 05). CONCLUSION The study suggested that sodium orthovanadate can decrease the value of plasma glucose in fasting type 2 diabetes rats and that has a protective function for maltose tolerance curve.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2003年第3期322-325,共4页
Chinese Pharmacological Bulletin
基金
黑龙江省科技厅重大项目基金资助课题No 2 0 0 10 10 10 0 1-0 0
关键词
原钒酸钠
链脲菌素
糖尿病
降糖作用
sodium orthovanadate
streptozocin
diabetes
hypoglycemic action
