摘要
目的 合成有生物活性的胸腺五肽并研究其免疫增强作用。方法 用Fmoc固相肽合成法合成胸腺五肽 ,用E玫瑰花实验检测生物活性。合成的胸腺五肽经小鼠腹腔注射后进行淋巴细胞转化实验、溶血空斑实验及IL 2活性实验 ,研究其免疫增强作用。结果 合成 2批胸腺五肽 ,精肽产量分别为 2 7mg和 3 4mg ,具有生物活性 ,实验组动物脾淋巴细胞的转化率、空斑形成细胞及IL 2活性皆明显高于空白对照组。结论 用Fmoc固相肽合成法可以成功地合成有生物活性的胸腺五肽 ,且具有免疫增强作用。
Objective To synthesize thymopoietin 5 with biological activity and study the immunopotentiation of it.Methods Synthesize thymopoietin 5 by Fmoc solid phase peptide synthesis and detect biological activity by E rosette test.Inject i.d. the synthetic thymopoietin 5 into mice and study the immunopotentiation of the drug by lymphocyte transformation,hemolytic plaque formation and IL 2 activity tests.Results Two batches of thymopoietin 5 with biological activity,at the yields of 27 and 34mg respectively,were synthesized.The spleen lymphocyte transformation rate,count of plaque forming cell,as well as IL 2 activity of test group,were significantly higher than those of control group.Conclusion Thymopoietin 5 was successfully synthesized by Fmoc solid phase peptide synthesis and showed immunopotentiation.
出处
《中国生物制品学杂志》
CAS
CSCD
2003年第2期87-89,共3页
Chinese Journal of Biologicals
关键词
胸腺五肽
固相肽合成
生物活性
免疫增强作用
Thymopoietin Solid phase peptide synthesis Biological activity Immunopotentiation
