摘要
目的:探讨nm23H1基因与胃癌细胞体内外侵袭力的关系.方法:采用Boyden小室法测定四种胃癌细胞系MKN1、MKN45、GT3TKB、BGC823的体外侵袭力,Northern印记杂交,RT-PCR及免疫组化技术检测胃癌组织、四种胃癌细胞系的nm23H1mRNA及蛋白表达水平.结果:胃癌细胞体外侵袭力高低的顺序依次为MKN45最高(33.1±5.2,与其他三种细胞系相比P<0.01);BGC823(15.8±2.7),MKN1(14.1±4.5)次之(二者无显著差异,P>0.05),GT3TKB最低(6.3±2.5,与其他三种细胞系相比P<0.01).nm23H1基因表达与胃癌细胞体内侵袭能力呈反比关系;nm23H1基因表达与MKN45,BGC823,GT3TKB细胞体外侵袭力也呈反比关系,但与MKN1细胞体外侵袭力无明显关系.结论:nm23H1表达在评价胃癌细胞体内外侵袭力方面具有重要价值.
AIM:To explore the relationship between the expression of nm23H1 gene and the in vivo and in vitro.invasive capacity of gastric cancer cells METHODS:The invasive capacity of gastric cancer cell in vitro was determined by Boyden chamber method. And the expressions of nm23H1 in gene and protein level were mea- sured by Northern Blot, RT-PCR and immunohistochemical method in gastric cancer cell lines, respectively. RESULTS:The order of the invasive capacity of gastric can- cer cell lines were: MKN45, being the highest (33.1±5.23, P <0.01); BGC823 (15.8±2.7) and MKN1 (14.1±4.5),the moderate (there was no significant difference between them, P >0.05), and GT3TKB(6.3±2.5), the lowest (P <0.01). The expression of nm23H1 gene was negatively correlated with the in vivo invasive capacity of gastric cancer cell lines, and also negatively correlated with the in vitro invasive capacity of gastric cancer cell lines of MKN45, BGC823 and GT3TKB. But there was no relationship between the expression of nm23H1 gene and the invasive capacity of MKN1 in vitro. CONCLUSION: The expression of nm23H1 gene is of great significance in evaluating the in vivo and in vitro invasive capacity of gastric cancer cell lines.
出处
《世界华人消化杂志》
CAS
2003年第1期10-13,共4页
World Chinese Journal of Digestology
基金
国家"973"项目(国家重点基础研究发展规划项目")恶性肿瘤发生与发展的基础性研究"子项目课题
No.G1998051203~~
